A case of peritoneal dialysis-associated peritonitis caused by Rhodococcus kroppenstedtii
Abstract Background Rhodococcus kroppenstedtii is an aerobic, gram-positive bacterium firstly identified in the environment, which has not been reported in human-related infection. Herein, we reported the first case of peritoneal dialysis (PD)-associated peritonitis caused by R. kroppenstedtii which...
Main Authors: | , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2021-06-01
|
Series: | BMC Infectious Diseases |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12879-021-06280-8 |
id |
doaj-b21d2896f6474d1698c569b1a34df6d6 |
---|---|
record_format |
Article |
spelling |
doaj-b21d2896f6474d1698c569b1a34df6d62021-06-20T11:07:58ZengBMCBMC Infectious Diseases1471-23342021-06-012111610.1186/s12879-021-06280-8A case of peritoneal dialysis-associated peritonitis caused by Rhodococcus kroppenstedtiiYi Kang0Yuxin Chen1Zhifeng Zhang2Han Shen3Wanqing Zhou4Chao Wu5Department of Infectious Diseases, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical UniversityDepartment of Laboratory Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical SchoolDepartment of Laboratory Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical SchoolDepartment of Laboratory Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical SchoolDepartment of Laboratory Medicine, Nanjing Drum Tower Hospital, the Affiliated Hospital of Nanjing University Medical SchoolDepartment of Infectious Diseases, Nanjing Drum Tower Hospital, Clinical College of Nanjing Medical UniversityAbstract Background Rhodococcus kroppenstedtii is an aerobic, gram-positive bacterium firstly identified in the environment, which has not been reported in human-related infection. Herein, we reported the first case of peritoneal dialysis (PD)-associated peritonitis caused by R. kroppenstedtii which was identified by whole genome sequencing. Case presentation A 69-year-old man was admitted to hospital with abdominal pain and fever. Over the last 2 years, he had been undergoing continuous ambulatory peritoneal dialysis (CAPD) due to end-stage renal disease. Clinical symptom and sign in combination with laboratory examinations supported the clinical diagnosis of PD-associated peritonitis. Thus, ceftizoxime and teicoplanin were empirically used after PD effluent was collected for bacterial culture. A gram-positive bacterium was found from the PD effluent culture, which could not be identified by either Vitek 2 Compact ANC card or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The strain was finally confirmed to be R. kroppenstedtii by whole genome sequencing (WGS) through the average nucleotide identity (ANI) analysis. With a continuous treatment with teicoplanin and imipenem for 15 days and intraperitoneal catheter removed, the infection symptom was improved evidenced by a normal body temperature, also with white blood cell count (WBC), procalcitonin (PCT) and C-reactive protein (CRP) dropped to normal levels. Peritoneal dialysis effluent culture showed a negative result. Then, hemodialysis and arteriovenous fistula angioplasty were performed, but the patient developed a progressive blood pressure loss, accompanied by multiple organ disorder, and died on Feb 25, 2020. Conclusions To the best of our knowledge, this is the first time to report a peritoneal dialysis-associated peritonitis caused by R. kroppenstedtii which was identified by average nucleotide identity analysis based on WGS.https://doi.org/10.1186/s12879-021-06280-8Rhodococcus kroppenstedtiiPeritoneal dialysis-associated peritonitisWhole genome sequencingAverage nucleotide identity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yi Kang Yuxin Chen Zhifeng Zhang Han Shen Wanqing Zhou Chao Wu |
spellingShingle |
Yi Kang Yuxin Chen Zhifeng Zhang Han Shen Wanqing Zhou Chao Wu A case of peritoneal dialysis-associated peritonitis caused by Rhodococcus kroppenstedtii BMC Infectious Diseases Rhodococcus kroppenstedtii Peritoneal dialysis-associated peritonitis Whole genome sequencing Average nucleotide identity |
author_facet |
Yi Kang Yuxin Chen Zhifeng Zhang Han Shen Wanqing Zhou Chao Wu |
author_sort |
Yi Kang |
title |
A case of peritoneal dialysis-associated peritonitis caused by Rhodococcus kroppenstedtii |
title_short |
A case of peritoneal dialysis-associated peritonitis caused by Rhodococcus kroppenstedtii |
title_full |
A case of peritoneal dialysis-associated peritonitis caused by Rhodococcus kroppenstedtii |
title_fullStr |
A case of peritoneal dialysis-associated peritonitis caused by Rhodococcus kroppenstedtii |
title_full_unstemmed |
A case of peritoneal dialysis-associated peritonitis caused by Rhodococcus kroppenstedtii |
title_sort |
case of peritoneal dialysis-associated peritonitis caused by rhodococcus kroppenstedtii |
publisher |
BMC |
series |
BMC Infectious Diseases |
issn |
1471-2334 |
publishDate |
2021-06-01 |
description |
Abstract Background Rhodococcus kroppenstedtii is an aerobic, gram-positive bacterium firstly identified in the environment, which has not been reported in human-related infection. Herein, we reported the first case of peritoneal dialysis (PD)-associated peritonitis caused by R. kroppenstedtii which was identified by whole genome sequencing. Case presentation A 69-year-old man was admitted to hospital with abdominal pain and fever. Over the last 2 years, he had been undergoing continuous ambulatory peritoneal dialysis (CAPD) due to end-stage renal disease. Clinical symptom and sign in combination with laboratory examinations supported the clinical diagnosis of PD-associated peritonitis. Thus, ceftizoxime and teicoplanin were empirically used after PD effluent was collected for bacterial culture. A gram-positive bacterium was found from the PD effluent culture, which could not be identified by either Vitek 2 Compact ANC card or matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The strain was finally confirmed to be R. kroppenstedtii by whole genome sequencing (WGS) through the average nucleotide identity (ANI) analysis. With a continuous treatment with teicoplanin and imipenem for 15 days and intraperitoneal catheter removed, the infection symptom was improved evidenced by a normal body temperature, also with white blood cell count (WBC), procalcitonin (PCT) and C-reactive protein (CRP) dropped to normal levels. Peritoneal dialysis effluent culture showed a negative result. Then, hemodialysis and arteriovenous fistula angioplasty were performed, but the patient developed a progressive blood pressure loss, accompanied by multiple organ disorder, and died on Feb 25, 2020. Conclusions To the best of our knowledge, this is the first time to report a peritoneal dialysis-associated peritonitis caused by R. kroppenstedtii which was identified by average nucleotide identity analysis based on WGS. |
topic |
Rhodococcus kroppenstedtii Peritoneal dialysis-associated peritonitis Whole genome sequencing Average nucleotide identity |
url |
https://doi.org/10.1186/s12879-021-06280-8 |
work_keys_str_mv |
AT yikang acaseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT yuxinchen acaseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT zhifengzhang acaseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT hanshen acaseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT wanqingzhou acaseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT chaowu acaseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT yikang caseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT yuxinchen caseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT zhifengzhang caseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT hanshen caseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT wanqingzhou caseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii AT chaowu caseofperitonealdialysisassociatedperitonitiscausedbyrhodococcuskroppenstedtii |
_version_ |
1721370483112280064 |