Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)

Background: Injury by the sting of Lesser weever fish (Trachinus vipera) may lead to severe pain, edema or tissue necrosis. Cellular effects of the venom are still incompletely understood. Previous observations revealed that purified Lesser weever fish venom (LWFV) induces suicidal death of erythroc...

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Main Authors: Myriam Fezai, Chaker Slaymi, Mossadok Ben-Attia, Guido Kroemer, Florian Lang, Mohamed Jemaà
Format: Article
Language:English
Published: Cell Physiol Biochem Press GmbH & Co KG 2017-04-01
Series:Cellular Physiology and Biochemistry
Subjects:
p53
Online Access:http://www.karger.com/Article/FullText/475646
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spelling doaj-b1fc633ae3d94f80bef081dbebeb63c72020-11-25T00:49:46ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-04-014162279228810.1159/000475646475646Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)Myriam FezaiChaker SlaymiMossadok Ben-AttiaGuido KroemerFlorian LangMohamed JemaàBackground: Injury by the sting of Lesser weever fish (Trachinus vipera) may lead to severe pain, edema or tissue necrosis. Cellular effects of the venom are still incompletely understood. Previous observations revealed that purified Lesser weever fish venom (LWFV) induces suicidal death of erythrocytes and HCT116 human colon carcinoma cells. The present study addressed the effect of the venom on colon carcinoma cell toxicity, shape and migration both in p53+/+ and/or p53-/- conditions. Methods: Cells were exposed to medium without or with 500 µg/ ml LWFV. Cell shape, cell area and circularity were visualized and quantified by fluorescence microscopy. Cell volume, granularity and cells toxicity were assessed via the apoptotic parameters dissipation of mitochondrial inner transmembrane potential, phosphatidylserine surface exposure and cell membrane permeabilization were measured utilizing flow cytometry. Cell migration was evaluated using wound healing assay and two-dimensional migration assay. Results: LWFV treatment was followed by a marked change of cell shape and size, significant decrease of cell area and circularity, significant impairment of cell migration, as well as induction of apoptosis after long exposition. Conclusions: LWFV exposure leads to cell shrinkage, increased granularity, apoptosis and impairment of cell migration, effects presumably contributing to LWFV-induced tissue injury.http://www.karger.com/Article/FullText/475646Lesser weever fish venomCell volumeGranularityMigrationMitochondrial pathway of apoptosisp53
collection DOAJ
language English
format Article
sources DOAJ
author Myriam Fezai
Chaker Slaymi
Mossadok Ben-Attia
Guido Kroemer
Florian Lang
Mohamed Jemaà
spellingShingle Myriam Fezai
Chaker Slaymi
Mossadok Ben-Attia
Guido Kroemer
Florian Lang
Mohamed Jemaà
Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)
Cellular Physiology and Biochemistry
Lesser weever fish venom
Cell volume
Granularity
Migration
Mitochondrial pathway of apoptosis
p53
author_facet Myriam Fezai
Chaker Slaymi
Mossadok Ben-Attia
Guido Kroemer
Florian Lang
Mohamed Jemaà
author_sort Myriam Fezai
title Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)
title_short Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)
title_full Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)
title_fullStr Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)
title_full_unstemmed Inhibition of Colon Carcinoma Cell Migration Following Treatment with Purified Venom from Lesser Weever Fish (Trachinus Vipera)
title_sort inhibition of colon carcinoma cell migration following treatment with purified venom from lesser weever fish (trachinus vipera)
publisher Cell Physiol Biochem Press GmbH & Co KG
series Cellular Physiology and Biochemistry
issn 1015-8987
1421-9778
publishDate 2017-04-01
description Background: Injury by the sting of Lesser weever fish (Trachinus vipera) may lead to severe pain, edema or tissue necrosis. Cellular effects of the venom are still incompletely understood. Previous observations revealed that purified Lesser weever fish venom (LWFV) induces suicidal death of erythrocytes and HCT116 human colon carcinoma cells. The present study addressed the effect of the venom on colon carcinoma cell toxicity, shape and migration both in p53+/+ and/or p53-/- conditions. Methods: Cells were exposed to medium without or with 500 µg/ ml LWFV. Cell shape, cell area and circularity were visualized and quantified by fluorescence microscopy. Cell volume, granularity and cells toxicity were assessed via the apoptotic parameters dissipation of mitochondrial inner transmembrane potential, phosphatidylserine surface exposure and cell membrane permeabilization were measured utilizing flow cytometry. Cell migration was evaluated using wound healing assay and two-dimensional migration assay. Results: LWFV treatment was followed by a marked change of cell shape and size, significant decrease of cell area and circularity, significant impairment of cell migration, as well as induction of apoptosis after long exposition. Conclusions: LWFV exposure leads to cell shrinkage, increased granularity, apoptosis and impairment of cell migration, effects presumably contributing to LWFV-induced tissue injury.
topic Lesser weever fish venom
Cell volume
Granularity
Migration
Mitochondrial pathway of apoptosis
p53
url http://www.karger.com/Article/FullText/475646
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