Lamination of microfibrous PLGA fabric by electrospinning a layer of collagen-hydroxyapatite composite nanofibers for bone tissue engineering

• Main Authors: Gi-Wan Kwon, Kailash Chandra Gupta, Kyung-Hye Jung, Inn-Kyu Kang
• Format: Article
• Language: English
• Published: BMC 2017-06-01
• Series: Biomaterials Research
• Subjects: Collagen; PLGA; Polydopamine; Hydroxyapatite nanorods and Scaffolds
• Online Access: http://link.springer.com/article/10.1186/s40824-017-0097-3

Abstract Background To mimic the muscle inspired cells adhesion through proteins secretion, the lamination of collagen–hydroxyapatite nanorod (nHA) composite nanofibers has been carried out successfully on polydopamine (PDA)-coated microfibrous polylactide-co-glycolide (PLGA) fabrics. The lamination of collagen-hydroxyapatite composite nanofibers on polydopamine-coated microfibrous PLGA fabrics was carried through electrospinning the solution of collagen containing L-glutamic acid-grafted hydroxyapatite nanorods (nHA-GA) at a flow rate of 1.5 mL/h and an applied voltage of 15 kV. Results In comparison to pristine PLGA, dopamine-coated PLGA and collagen-hydroxyapatite composite nanofiber lamination has produced more wettable surfaces and surface wettability is found to higher with dopamine-coated PLGA fabrics then pristine PLGA. The SEM micrographs have clearly indicated that the lamination of polydopamine-coated PLGA fabric with collagen-hydroxyapatite composite nanofibers has shown increased adhesion of MC3T3E1 cells in comparison to pristine PLGA fabrics. Conclusion The results of these studies have clearly demonstrated that collagen-nHA composites fibers may be used to create bioactive 3D scaffolds using PLGA as an architectural support agent.