The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method

Strawberry (Fragaria ananassa Duch.) is one of most important fruit crops cultivated widely in world. In the present study, we have investigated the proteome variation to different commercially available red strawberry varieties, to gain better insight into the variation in allergen content between...

Full description

Bibliographic Details
Main Authors:	Carmine Guarino, Rosaria Sciarrillo
Format:	Article
Language:	English
Published:	Elsevier 2018-12-01
Series:	Annals of Agricultural Sciences
Online Access:	http://www.sciencedirect.com/science/article/pii/S0570178318300319

id	doaj-b1e177f378c64a579b91f1f8fc8984c1
record_format	Article
spelling	doaj-b1e177f378c64a579b91f1f8fc8984c12020-11-24T23:58:42ZengElsevierAnnals of Agricultural Sciences0570-17832018-12-01632181189The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting methodCarmine Guarino0Rosaria Sciarrillo1University of Sannio, Department of Science and Technology, via Port’Arsa 11, 82100 Benevento, ItalyCorresponding author.; University of Sannio, Department of Science and Technology, via Port’Arsa 11, 82100 Benevento, ItalyStrawberry (Fragaria ananassa Duch.) is one of most important fruit crops cultivated widely in world. In the present study, we have investigated the proteome variation to different commercially available red strawberry varieties, to gain better insight into the variation in allergen content between the different varieties, as well as their proteome variation. In fact, a combination of proteomic tools have been used to build a complete allergen map of strawberry. The water-soluble fraction of a strawberry extract was precipitated using a phenol-based procedure and separated by 2-DE. Further, all known strawberry allergens were localized on a 2-DE map and they were matched with spots recognized by sera of patients with different allergic patterns. A number of constitutive or differentially accumulated proteins were found. The role of the identified proteins, in particular of the allergen proteins, is discussed in relation to the different variety of strawberry. Keywords: Proteomics, 2-DE, Fragaria ananassa, Mass spectrometry, Allergen proteinshttp://www.sciencedirect.com/science/article/pii/S0570178318300319
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Carmine Guarino Rosaria Sciarrillo
spellingShingle	Carmine Guarino Rosaria Sciarrillo The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method Annals of Agricultural Sciences
author_facet	Carmine Guarino Rosaria Sciarrillo
author_sort	Carmine Guarino
title	The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method
title_short	The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method
title_full	The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method
title_fullStr	The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method
title_full_unstemmed	The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method
title_sort	identification of allergen proteins in two different varieties of strawberry by two different approaches: proteomic and western blotting method
publisher	Elsevier
series	Annals of Agricultural Sciences
issn	0570-1783
publishDate	2018-12-01
description	Strawberry (Fragaria ananassa Duch.) is one of most important fruit crops cultivated widely in world. In the present study, we have investigated the proteome variation to different commercially available red strawberry varieties, to gain better insight into the variation in allergen content between the different varieties, as well as their proteome variation. In fact, a combination of proteomic tools have been used to build a complete allergen map of strawberry. The water-soluble fraction of a strawberry extract was precipitated using a phenol-based procedure and separated by 2-DE. Further, all known strawberry allergens were localized on a 2-DE map and they were matched with spots recognized by sera of patients with different allergic patterns. A number of constitutive or differentially accumulated proteins were found. The role of the identified proteins, in particular of the allergen proteins, is discussed in relation to the different variety of strawberry. Keywords: Proteomics, 2-DE, Fragaria ananassa, Mass spectrometry, Allergen proteins
url	http://www.sciencedirect.com/science/article/pii/S0570178318300319
work_keys_str_mv	AT carmineguarino theidentificationofallergenproteinsintwodifferentvarietiesofstrawberrybytwodifferentapproachesproteomicandwesternblottingmethod AT rosariasciarrillo theidentificationofallergenproteinsintwodifferentvarietiesofstrawberrybytwodifferentapproachesproteomicandwesternblottingmethod AT carmineguarino identificationofallergenproteinsintwodifferentvarietiesofstrawberrybytwodifferentapproachesproteomicandwesternblottingmethod AT rosariasciarrillo identificationofallergenproteinsintwodifferentvarietiesofstrawberrybytwodifferentapproachesproteomicandwesternblottingmethod
_version_	1725450154470801408

The identification of allergen proteins in two different varieties of strawberry by two different approaches: Proteomic and western blotting method

Similar Items