A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in Mice

A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in Mice

The maintenance of intestinal homeostasis is necessary for a good quality of life, and strengthening of the intestinal barrier function is thus an important issue. Therefore, we focused on soybean resistant protein (SRP) derived from kori-tofu (freeze-dried tofu), which is a traditional Japanese foo...

Full description

Bibliographic Details
Main Authors: Tasuku Ogita, Fu Namai, Ayane Mikami, Takahiro Ishiguro, Koji Umezawa, Yutaka Uyeno, Takeshi Shimosato
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-08-01
Series:Frontiers in Nutrition
Subjects:
cecal microbiota
intestinal barrier
PiCRUSt
Reg3γ
soybean resistant protein
Online Access:https://www.frontiersin.org/articles/10.3389/fnut.2021.701466/full
id doaj-b1dfd950945248b09437628b3c21e85b
record_format Article
spelling doaj-b1dfd950945248b09437628b3c21e85b2021-08-19T06:44:02ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2021-08-01810.3389/fnut.2021.701466701466A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in MiceTasuku Ogita0Fu Namai1Fu Namai2Ayane Mikami3Takahiro Ishiguro4Koji Umezawa5Yutaka Uyeno6Takeshi Shimosato7Department of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, JapanDepartment of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, JapanDepartment of Pathology, Graduate School of Biomedical Sciences, Nagasaki University, Nagasaki, JapanDepartment of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, JapanFood Research Center, Asahimatsu Foods Co., Ltd., Nagano, JapanDepartment of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, JapanFaculty of Agriculture, Shinshu University, Nagano, JapanDepartment of Biomolecular Innovation, Institute for Biomedical Sciences, Shinshu University, Nagano, JapanThe maintenance of intestinal homeostasis is necessary for a good quality of life, and strengthening of the intestinal barrier function is thus an important issue. Therefore, we focused on soybean resistant protein (SRP) derived from kori-tofu (freeze-dried tofu), which is a traditional Japanese food, as a functional food component. In this study, to investigate the effect of SRP on the intestinal barrier function and intestinal microbiota, we conducted an SRP free intake experiment in mice. Results showed that ingestion of SRP decreased the serum level of lipopolysaccharide-binding protein and induced the expression of Reg3γ, thereby improving the intestinal barrier function. In addition, SRP intake induced changes in the cecal microbiota, as observed by changes in β-diversity. In particular, in the microbiota, the up-regulation of functional gene pathways related to the bacterial invasion of epithelial cells (ko05100) was observed, suggesting that Reg3γ expression was induced by the direct stimulation of epithelial cells. The results of this study suggest that SRP is a functional food component that may contribute to the maintenance of intestinal homeostasis.https://www.frontiersin.org/articles/10.3389/fnut.2021.701466/fullcecal microbiotaintestinal barrierPiCRUStReg3γsoybean resistant protein
collection DOAJ
language English
format Article
sources DOAJ
author Tasuku Ogita
Fu Namai
Fu Namai
Ayane Mikami
Takahiro Ishiguro
Koji Umezawa
Yutaka Uyeno
Takeshi Shimosato
spellingShingle Tasuku Ogita
Fu Namai
Fu Namai
Ayane Mikami
Takahiro Ishiguro
Koji Umezawa
Yutaka Uyeno
Takeshi Shimosato
A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in Mice
Frontiers in Nutrition
cecal microbiota
intestinal barrier
PiCRUSt
Reg3γ
soybean resistant protein
author_facet Tasuku Ogita
Fu Namai
Fu Namai
Ayane Mikami
Takahiro Ishiguro
Koji Umezawa
Yutaka Uyeno
Takeshi Shimosato
author_sort Tasuku Ogita
title A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in Mice
title_short A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in Mice
title_full A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in Mice
title_fullStr A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in Mice
title_full_unstemmed A Soybean Resistant Protein-Containing Diet Increased the Production of Reg3γ Through the Regulation of the Gut Microbiota and Enhanced the Intestinal Barrier Function in Mice
title_sort soybean resistant protein-containing diet increased the production of reg3γ through the regulation of the gut microbiota and enhanced the intestinal barrier function in mice
publisher Frontiers Media S.A.
series Frontiers in Nutrition
issn 2296-861X
publishDate 2021-08-01
description The maintenance of intestinal homeostasis is necessary for a good quality of life, and strengthening of the intestinal barrier function is thus an important issue. Therefore, we focused on soybean resistant protein (SRP) derived from kori-tofu (freeze-dried tofu), which is a traditional Japanese food, as a functional food component. In this study, to investigate the effect of SRP on the intestinal barrier function and intestinal microbiota, we conducted an SRP free intake experiment in mice. Results showed that ingestion of SRP decreased the serum level of lipopolysaccharide-binding protein and induced the expression of Reg3γ, thereby improving the intestinal barrier function. In addition, SRP intake induced changes in the cecal microbiota, as observed by changes in β-diversity. In particular, in the microbiota, the up-regulation of functional gene pathways related to the bacterial invasion of epithelial cells (ko05100) was observed, suggesting that Reg3γ expression was induced by the direct stimulation of epithelial cells. The results of this study suggest that SRP is a functional food component that may contribute to the maintenance of intestinal homeostasis.
topic cecal microbiota
intestinal barrier
PiCRUSt
Reg3γ
soybean resistant protein
url https://www.frontiersin.org/articles/10.3389/fnut.2021.701466/full
work_keys_str_mv AT tasukuogita asoybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT funamai asoybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT funamai asoybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT ayanemikami asoybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT takahiroishiguro asoybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT kojiumezawa asoybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT yutakauyeno asoybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT takeshishimosato asoybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT tasukuogita soybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT funamai soybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT funamai soybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT ayanemikami soybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT takahiroishiguro soybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT kojiumezawa soybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT yutakauyeno soybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
AT takeshishimosato soybeanresistantproteincontainingdietincreasedtheproductionofreg3gthroughtheregulationofthegutmicrobiotaandenhancedtheintestinalbarrierfunctioninmice
_version_ 1721202551667294208

Similar Items