Evaluating the Association between Rs1800624 in RAGE Gene and Multiple Sclerosis in Isfahan Population

• Main Authors: A Jazaeri, A Karimi Moghadam, S Vallian Borujeni
• Format: Article
• Language: fas
• Published: Shahid Sadoughi University of Medical Sciences 2016-01-01
• Series: Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd
• Subjects: Multiple sclerosis; RAGE gene; Single nucleotide polymorphism
• Online Access: http://jssu.ssu.ac.ir/browse.php?a_code=A-10-1666-2&slc_lang=en&sid=1
• ISSN: 2228-5741; 2228-5733

Introduction: Multiple sclerosis (MS) is an acute disease of the central nervous system (CNS) associated with the degradation of myelin sheet around the nerve cells. It is assumed to be a multifactorial disorder that is to say numerous environmental and genetic factors are involved in the disease. Therefore, this study aimed to investigate the association between rs1800624 single nucleotide polymorphism (SNP), located in the promoter region of RAGE gene, and MS in Isfahan population. Methods: In this case-control study, tetra-primer ARMS PCR and newly designed primers were utilized in genotyping and determining allele frequency of rs1800624 SNP marker. A total of 300 individuals including 150 healthy and 150 MS patients participated in the study. In order to evaluate the association between genotypes and MS as well as the existence of Hardy-Weinberg equilibrium, SPSS and GENPOP softwares were used, respectively. Results: The study results showed that the frequency of TT, TA and AA genotypes were reported 42.7, 46.6 and 10.7 in the control group and 44, 42 and 14 in the case group. The studied population was in equilibrium in regard with rs1800624 marker. Moreover, no association was detected between rs1800624 marker and MS disease in the Isfahan population (p>0.5). Conclusion: The study findings indicated no association between SNP marker of rs1800624 and MS disease in the Isfahan population (p>0.5).