Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors

Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors

Abstract Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and f...

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Bibliographic Details
Main Authors: Meiling Zhang, Changyang Zhou, Yu Wei, Chunlong Xu, Hong Pan, Wenqin Ying, Yidi Sun, Yun Sun, Qingquan Xiao, Ning Yao, Wanxia Zhong, Yun Li, Keliang Wu, Gao Yuan, Shoukhrat Mitalipov, Zi-jiang Chen, Hui Yang
Format: Article
Language:English
Published: BMC 2019-05-01
Series:Genome Biology
Subjects:
Base editing
Human cleaving embryos
Homozygotic nucleotide substitution
Online Access:http://link.springer.com/article/10.1186/s13059-019-1703-6

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