Spectrophotometric Determination of L-Ascorbic Acid in Pharmaceuticals Based on Its Oxidation by Potassium Peroxymonosulfate and Hydrogen Peroxide
Two new, accurate, precise, and sensitive spectrophotometric methods were developed for the assay of L-ascorbic acid in pharmaceutical preparations. The determination of L-ascorbic acid was based on its oxidation by potassium peroxymonosulfate (method A) and hydrogen peroxide in the presence of C...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Croatian Chemical Society
2015-03-01
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| Series: | Croatica Chemica Acta |
| Online Access: | http://hrcak.srce.hr/file/205143 |
| Summary: | Two new, accurate, precise, and sensitive spectrophotometric methods were developed for
the assay of L-ascorbic acid in pharmaceutical preparations. The determination of L-ascorbic acid was
based on its oxidation by potassium peroxymonosulfate (method A) and hydrogen peroxide in the
presence of Cu(II) as a catalyst (method B). The molar absorptivities were found to be 1.48×104 and
1.06×104 L mol–1 cm–1 for methods A and B, respectively. Beer's law was obeyed in the concentration
range of 0.65–11.20 μg mL–1 for method A and 0.51–16.00 μg mL–1 for method B. Other compounds
commonly found in vitamin C and multivitamin products did not interfere with the determination of
L-ascorbic acid.. The proposed methods were successfully applied for the determination of L-ascorbic acid
in pharmaceutical formulations. The results obtained with the proposed methods showed good agreement
with those given by the titrimetric method using iodine. |
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| ISSN: | 0011-1643 1334-417X |