ESTABELECIMENTO DE UM PROTOCOLO DE SOROAGLUTINAÇÃO RÁPIDA (SAR) PARA DETECÇÃO DE ANTICORPOS PARA Salmonella Typhimurium EM SUÍNOS

• Main Authors: Rosecler Alves Pereirara, Mararisa Macagnan, Patricia Schwarz, C laudio Wageck Canal, Verônica Schmidt
• Format: Article
• Language: English
• Published: Universidade Federal de Goiás 2010-10-01
• Series: Ciência Animal Brasileira
• Subjects: Antibody; diagnosis; pig; rapid agglutination test; Salmonella Typhimurium.
• Online Access: http://www.revistas.ufg.br/index.php/vet/article/view/6100/7937

Salmonella Typhimurium is an important agent isolated from cases of human food poisoning in Brazil. Animal-origin products are the main source of infection, and pork has been implicated in Salmonella transmission to humans. Programs to salmonella monitoring in swine have been carried out, using bacteriologicaland serological tests like ELISA. However, these tests are time-consuming and expensive. The rapid agglutination test (RAT) is cheaper, faster and easier. Aiming to standardize RAT to detect anti-salmonella antibodies in swine serum, 60 samples of swine serum were tested. They had previously displayed positive (30) or negative (30) results in the ELISA test. The results showed that RAT had sensitivity, specificity, predictive positive value and predictive negative value equal to 96.7% when non-diluted serum was tested. Thus, this test can be applied to detect antibodies against S. Typhimurium in swine serum.