Interleukin-1 Modulation of Human Placental Trophoblast Proliferation

During early pregnancy, interleukin-1 (IL-1) is mainly produced and secreted by maternal decidua. Yet, its biological function on placental cells is not well defined. In this study, we employed JAR choriocarcinoma cell line as a model of human placental trophoblast to study the effect of IL-1. Treat...

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Main Authors: Athip Nilkaeo, Suthinee Bhuvanath
Format: Article
Language:English
Published: Hindawi Limited 2006-01-01
Series:Mediators of Inflammation
Online Access:http://dx.doi.org/10.1155/MI/2006/79359
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spelling doaj-b1605af423bc41f69d0082426e96ba612020-11-24T21:02:53ZengHindawi LimitedMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/7935979359Interleukin-1 Modulation of Human Placental Trophoblast ProliferationAthip Nilkaeo0Suthinee Bhuvanath1Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla 90112, ThailandDepartment of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla 90112, ThailandDuring early pregnancy, interleukin-1 (IL-1) is mainly produced and secreted by maternal decidua. Yet, its biological function on placental cells is not well defined. In this study, we employed JAR choriocarcinoma cell line as a model of human placental trophoblast to study the effect of IL-1. Treatment with recombinant human IL-1β resulted in significant inhibition of JAR proliferation (P<.05) paralleled with increased cytotoxicity. The inhibitory effect was blocked by both IL-1 receptor antagonist (IL-1Ra) and antihuman IL-1β monoclonal antibody. Analyzing the mode of action, IL-1β was found to induce cell cycle arrest in the G0/G1 phase and triggered apoptotic cell death. These findings demonstrated that IL-1 regulates human trophoblast growth by induction of cell cycle delay and cell death.http://dx.doi.org/10.1155/MI/2006/79359
collection DOAJ
language English
format Article
sources DOAJ
author Athip Nilkaeo
Suthinee Bhuvanath
spellingShingle Athip Nilkaeo
Suthinee Bhuvanath
Interleukin-1 Modulation of Human Placental Trophoblast Proliferation
Mediators of Inflammation
author_facet Athip Nilkaeo
Suthinee Bhuvanath
author_sort Athip Nilkaeo
title Interleukin-1 Modulation of Human Placental Trophoblast Proliferation
title_short Interleukin-1 Modulation of Human Placental Trophoblast Proliferation
title_full Interleukin-1 Modulation of Human Placental Trophoblast Proliferation
title_fullStr Interleukin-1 Modulation of Human Placental Trophoblast Proliferation
title_full_unstemmed Interleukin-1 Modulation of Human Placental Trophoblast Proliferation
title_sort interleukin-1 modulation of human placental trophoblast proliferation
publisher Hindawi Limited
series Mediators of Inflammation
issn 0962-9351
1466-1861
publishDate 2006-01-01
description During early pregnancy, interleukin-1 (IL-1) is mainly produced and secreted by maternal decidua. Yet, its biological function on placental cells is not well defined. In this study, we employed JAR choriocarcinoma cell line as a model of human placental trophoblast to study the effect of IL-1. Treatment with recombinant human IL-1β resulted in significant inhibition of JAR proliferation (P<.05) paralleled with increased cytotoxicity. The inhibitory effect was blocked by both IL-1 receptor antagonist (IL-1Ra) and antihuman IL-1β monoclonal antibody. Analyzing the mode of action, IL-1β was found to induce cell cycle arrest in the G0/G1 phase and triggered apoptotic cell death. These findings demonstrated that IL-1 regulates human trophoblast growth by induction of cell cycle delay and cell death.
url http://dx.doi.org/10.1155/MI/2006/79359
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AT suthineebhuvanath interleukin1modulationofhumanplacentaltrophoblastproliferation
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