Interleukin-1 Modulation of Human Placental Trophoblast Proliferation
During early pregnancy, interleukin-1 (IL-1) is mainly produced and secreted by maternal decidua. Yet, its biological function on placental cells is not well defined. In this study, we employed JAR choriocarcinoma cell line as a model of human placental trophoblast to study the effect of IL-1. Treat...
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Series: | Mediators of Inflammation |
Online Access: | http://dx.doi.org/10.1155/MI/2006/79359 |
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doaj-b1605af423bc41f69d0082426e96ba612020-11-24T21:02:53ZengHindawi LimitedMediators of Inflammation0962-93511466-18612006-01-01200610.1155/MI/2006/7935979359Interleukin-1 Modulation of Human Placental Trophoblast ProliferationAthip Nilkaeo0Suthinee Bhuvanath1Department of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla 90112, ThailandDepartment of Microbiology, Faculty of Science, Prince of Songkla University, Hat Yai, Songkla 90112, ThailandDuring early pregnancy, interleukin-1 (IL-1) is mainly produced and secreted by maternal decidua. Yet, its biological function on placental cells is not well defined. In this study, we employed JAR choriocarcinoma cell line as a model of human placental trophoblast to study the effect of IL-1. Treatment with recombinant human IL-1β resulted in significant inhibition of JAR proliferation (P<.05) paralleled with increased cytotoxicity. The inhibitory effect was blocked by both IL-1 receptor antagonist (IL-1Ra) and antihuman IL-1β monoclonal antibody. Analyzing the mode of action, IL-1β was found to induce cell cycle arrest in the G0/G1 phase and triggered apoptotic cell death. These findings demonstrated that IL-1 regulates human trophoblast growth by induction of cell cycle delay and cell death.http://dx.doi.org/10.1155/MI/2006/79359 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Athip Nilkaeo Suthinee Bhuvanath |
spellingShingle |
Athip Nilkaeo Suthinee Bhuvanath Interleukin-1 Modulation of Human Placental Trophoblast Proliferation Mediators of Inflammation |
author_facet |
Athip Nilkaeo Suthinee Bhuvanath |
author_sort |
Athip Nilkaeo |
title |
Interleukin-1 Modulation of Human Placental Trophoblast Proliferation |
title_short |
Interleukin-1 Modulation of Human Placental Trophoblast Proliferation |
title_full |
Interleukin-1 Modulation of Human Placental Trophoblast Proliferation |
title_fullStr |
Interleukin-1 Modulation of Human Placental Trophoblast Proliferation |
title_full_unstemmed |
Interleukin-1 Modulation of Human Placental Trophoblast Proliferation |
title_sort |
interleukin-1 modulation of human placental trophoblast proliferation |
publisher |
Hindawi Limited |
series |
Mediators of Inflammation |
issn |
0962-9351 1466-1861 |
publishDate |
2006-01-01 |
description |
During early pregnancy, interleukin-1 (IL-1) is mainly produced
and secreted by maternal decidua. Yet, its biological function on
placental cells is not well defined. In this study, we employed
JAR choriocarcinoma cell line as a model of human placental
trophoblast to study the effect of IL-1. Treatment with
recombinant human IL-1β resulted in significant inhibition
of JAR proliferation (P<.05) paralleled with increased
cytotoxicity. The inhibitory effect was blocked by both IL-1
receptor antagonist (IL-1Ra) and antihuman IL-1β monoclonal antibody. Analyzing the mode of action, IL-1β was found to induce cell cycle arrest in the G0/G1 phase and triggered
apoptotic cell death. These findings demonstrated that IL-1
regulates human trophoblast growth by induction of cell cycle
delay and cell death. |
url |
http://dx.doi.org/10.1155/MI/2006/79359 |
work_keys_str_mv |
AT athipnilkaeo interleukin1modulationofhumanplacentaltrophoblastproliferation AT suthineebhuvanath interleukin1modulationofhumanplacentaltrophoblastproliferation |
_version_ |
1716775067660058624 |