Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater
The use of honey as a remedy for microbial infections has been the reason behind recent researches on its antimicrobial activity. The research assessed the antibacterial activity of honey on <em>Eschericia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae </em>isolated from environm...
Main Authors: | , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Faculty of Science, University of Peradeniya, Sri Lanka
2020-12-01
|
Series: | Ceylon Journal of Science |
Subjects: | |
Online Access: | https://cjs.sljol.info/articles/7827 |
id |
doaj-b15db0dd1e9d4f368cb5788a5b9845b8 |
---|---|
record_format |
Article |
spelling |
doaj-b15db0dd1e9d4f368cb5788a5b9845b82021-09-20T15:23:31ZengFaculty of Science, University of Peradeniya, Sri LankaCeylon Journal of Science2513-28142513-230X2020-12-0149447147610.4038/cjs.v49i4.78275841Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewaterT. O. Agbabiaka0O. J. Awelogun1F. O. Otuyelu2T. O. Agbabiaka3University of Ilorin, P.M.B. 1515, IlorinUniversity of Ilorin, P.M.B. 1515, IlorinUniversity of Ilorin, P.M.B. 1515, IlorinUniversity of Ilorin, P.M.B. 1515, IlorinThe use of honey as a remedy for microbial infections has been the reason behind recent researches on its antimicrobial activity. The research assessed the antibacterial activity of honey on <em>Eschericia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae </em>isolated from environmental wastewater, using disc diffusion method at various concentrations of honey ranging from 62.5 - 1000 mg/ml while the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined using macro-dilution method. The zones of inhibition across the disc were measured after 24 hours of incubation. Results showed that honey has higher antibacterial activity on <em>E. coli </em>compared to other test isolates and also higher on <em>E. coli </em>than ciprofloxacin. Honey showed weaker activity on <em>K. pneumoniae </em>and <em>P. aeruginosa </em>compared to standard antibiotics. MIC was 250 mg/ml for <em>E. coli </em>while <em>K. pneumoniae </em>and <em>P. aeruginosa </em>were at 500 mg/ml. MBC for <em>E. coli, K. pneumoniae </em>and <em>P. aeruginosa </em>were observed at 312. 5 mg/ml, 687.5 mg/ml and 750 mg/ml respectively. Honey has promising antibacterial activity on infections caused by <em>E. coli, K. pneumoniae and P. aeruginosa </em>because of its antibacterial properties such as low pH, high osmolarity, and production of hydrogen peroxide.https://cjs.sljol.info/articles/7827disc diffusion, macrodilutio |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
T. O. Agbabiaka O. J. Awelogun F. O. Otuyelu T. O. Agbabiaka |
spellingShingle |
T. O. Agbabiaka O. J. Awelogun F. O. Otuyelu T. O. Agbabiaka Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater Ceylon Journal of Science disc diffusion, macrodilutio |
author_facet |
T. O. Agbabiaka O. J. Awelogun F. O. Otuyelu T. O. Agbabiaka |
author_sort |
T. O. Agbabiaka |
title |
Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater |
title_short |
Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater |
title_full |
Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater |
title_fullStr |
Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater |
title_full_unstemmed |
Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater |
title_sort |
antibacterial activity of honey (<em>apis mellifera</em>) on <em>pseudomonas aeruginosa, klebsiella pneumonia</em> and <em>escherichia coli</em> isolated from wastewater |
publisher |
Faculty of Science, University of Peradeniya, Sri Lanka |
series |
Ceylon Journal of Science |
issn |
2513-2814 2513-230X |
publishDate |
2020-12-01 |
description |
The use of honey as a remedy for microbial infections has been the reason behind recent researches on its antimicrobial activity. The research assessed the antibacterial activity of honey on <em>Eschericia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae </em>isolated from environmental wastewater, using disc diffusion method at various concentrations of honey ranging from 62.5 - 1000 mg/ml while the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined using macro-dilution method. The zones of inhibition across the disc were measured after 24 hours of incubation. Results showed that honey has higher antibacterial activity on <em>E. coli </em>compared to other test isolates and also higher on <em>E. coli </em>than ciprofloxacin. Honey showed weaker activity on <em>K. pneumoniae </em>and <em>P. aeruginosa </em>compared to standard antibiotics. MIC was 250 mg/ml for <em>E. coli </em>while <em>K. pneumoniae </em>and <em>P. aeruginosa </em>were at 500 mg/ml. MBC for <em>E. coli, K. pneumoniae </em>and <em>P. aeruginosa </em>were observed at 312. 5 mg/ml, 687.5 mg/ml and 750 mg/ml respectively. Honey has promising antibacterial activity on infections caused by <em>E. coli, K. pneumoniae and P. aeruginosa </em>because of its antibacterial properties such as low pH, high osmolarity, and production of hydrogen peroxide. |
topic |
disc diffusion, macrodilutio |
url |
https://cjs.sljol.info/articles/7827 |
work_keys_str_mv |
AT toagbabiaka antibacterialactivityofhoneyemapismelliferaemonempseudomonasaeruginosaklebsiellapneumoniaemandemescherichiacoliemisolatedfromwastewater AT ojawelogun antibacterialactivityofhoneyemapismelliferaemonempseudomonasaeruginosaklebsiellapneumoniaemandemescherichiacoliemisolatedfromwastewater AT footuyelu antibacterialactivityofhoneyemapismelliferaemonempseudomonasaeruginosaklebsiellapneumoniaemandemescherichiacoliemisolatedfromwastewater AT toagbabiaka antibacterialactivityofhoneyemapismelliferaemonempseudomonasaeruginosaklebsiellapneumoniaemandemescherichiacoliemisolatedfromwastewater |
_version_ |
1717374098009489408 |