Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater

The use of honey as a remedy for microbial infections has been the reason behind recent researches on its antimicrobial activity. The research assessed the antibacterial activity of honey on <em>Eschericia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae </em>isolated from environm...

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Main Authors: T. O. Agbabiaka, O. J. Awelogun, F. O. Otuyelu
Format: Article
Language:English
Published: Faculty of Science, University of Peradeniya, Sri Lanka 2020-12-01
Series:Ceylon Journal of Science
Subjects:
Online Access:https://cjs.sljol.info/articles/7827
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spelling doaj-b15db0dd1e9d4f368cb5788a5b9845b82021-09-20T15:23:31ZengFaculty of Science, University of Peradeniya, Sri LankaCeylon Journal of Science2513-28142513-230X2020-12-0149447147610.4038/cjs.v49i4.78275841Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewaterT. O. Agbabiaka0O. J. Awelogun1F. O. Otuyelu2T. O. Agbabiaka3University of Ilorin, P.M.B. 1515, IlorinUniversity of Ilorin, P.M.B. 1515, IlorinUniversity of Ilorin, P.M.B. 1515, IlorinUniversity of Ilorin, P.M.B. 1515, IlorinThe use of honey as a remedy for microbial infections has been the reason behind recent researches on its antimicrobial activity. The research assessed the antibacterial activity of honey on <em>Eschericia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae </em>isolated from environmental wastewater, using disc diffusion method at various concentrations of honey ranging from 62.5 - 1000 mg/ml while the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined using macro-dilution method. The zones of inhibition across the disc were measured after 24 hours of incubation. Results showed that honey has higher antibacterial activity on <em>E. coli </em>compared to other test isolates and also higher on <em>E. coli </em>than ciprofloxacin. Honey showed weaker activity on <em>K. pneumoniae </em>and <em>P. aeruginosa </em>compared to standard antibiotics. MIC was 250 mg/ml for <em>E. coli </em>while <em>K. pneumoniae </em>and <em>P. aeruginosa </em>were at 500 mg/ml. MBC for <em>E. coli, K. pneumoniae </em>and <em>P. aeruginosa </em>were observed at 312. 5 mg/ml, 687.5 mg/ml and 750 mg/ml respectively. Honey has promising antibacterial activity on infections caused by <em>E. coli, K. pneumoniae and P. aeruginosa </em>because of its antibacterial properties such as low pH, high osmolarity, and production of hydrogen peroxide.https://cjs.sljol.info/articles/7827disc diffusion, macrodilutio
collection DOAJ
language English
format Article
sources DOAJ
author T. O. Agbabiaka
O. J. Awelogun
F. O. Otuyelu
T. O. Agbabiaka
spellingShingle T. O. Agbabiaka
O. J. Awelogun
F. O. Otuyelu
T. O. Agbabiaka
Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater
Ceylon Journal of Science
disc diffusion, macrodilutio
author_facet T. O. Agbabiaka
O. J. Awelogun
F. O. Otuyelu
T. O. Agbabiaka
author_sort T. O. Agbabiaka
title Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater
title_short Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater
title_full Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater
title_fullStr Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater
title_full_unstemmed Antibacterial activity of honey (<em>Apis mellifera</em>) on <em>Pseudomonas aeruginosa, Klebsiella pneumonia</em> and <em>Escherichia coli</em> isolated from wastewater
title_sort antibacterial activity of honey (<em>apis mellifera</em>) on <em>pseudomonas aeruginosa, klebsiella pneumonia</em> and <em>escherichia coli</em> isolated from wastewater
publisher Faculty of Science, University of Peradeniya, Sri Lanka
series Ceylon Journal of Science
issn 2513-2814
2513-230X
publishDate 2020-12-01
description The use of honey as a remedy for microbial infections has been the reason behind recent researches on its antimicrobial activity. The research assessed the antibacterial activity of honey on <em>Eschericia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae </em>isolated from environmental wastewater, using disc diffusion method at various concentrations of honey ranging from 62.5 - 1000 mg/ml while the minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined using macro-dilution method. The zones of inhibition across the disc were measured after 24 hours of incubation. Results showed that honey has higher antibacterial activity on <em>E. coli </em>compared to other test isolates and also higher on <em>E. coli </em>than ciprofloxacin. Honey showed weaker activity on <em>K. pneumoniae </em>and <em>P. aeruginosa </em>compared to standard antibiotics. MIC was 250 mg/ml for <em>E. coli </em>while <em>K. pneumoniae </em>and <em>P. aeruginosa </em>were at 500 mg/ml. MBC for <em>E. coli, K. pneumoniae </em>and <em>P. aeruginosa </em>were observed at 312. 5 mg/ml, 687.5 mg/ml and 750 mg/ml respectively. Honey has promising antibacterial activity on infections caused by <em>E. coli, K. pneumoniae and P. aeruginosa </em>because of its antibacterial properties such as low pH, high osmolarity, and production of hydrogen peroxide.
topic disc diffusion, macrodilutio
url https://cjs.sljol.info/articles/7827
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