A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)

Cedrus deodara (deodar) is practically used, as insect repellent, in the northern areas of Pakistan but no data available therefore this study was conducted to evaluate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (Tene...

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Main Authors: Islam Dad Buneri, Masarrat Yousuf, Mohammad Attaullah, Saifullah Afridi, Syed Ishtiaq Anjum, Habibullah Rana, Naveed Ahmad, Muhammad Amin, Muhammad Tahir, Mohammad Javed Ansari
Format: Article
Language:English
Published: Elsevier 2019-02-01
Series:Saudi Journal of Biological Sciences
Online Access:http://www.sciencedirect.com/science/article/pii/S1319562X17301547
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spelling doaj-b157c64cd66b4cc4a74fdde2399927512020-11-25T00:30:59ZengElsevierSaudi Journal of Biological Sciences1319-562X2019-02-01262281285A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)Islam Dad Buneri0Masarrat Yousuf1Mohammad Attaullah2Saifullah Afridi3Syed Ishtiaq Anjum4Habibullah Rana5Naveed Ahmad6Muhammad Amin7Muhammad Tahir8Mohammad Javed Ansari9Toxicology Laboratory, Department of Zoology, University of Karachi, Karachi, PakistanToxicology Laboratory, Department of Zoology, University of Karachi, Karachi, PakistanDepartment of Zoology, Shaheed BB University, Sheringal, Upper Dir, PakistanCentre for Advanced Drug Research, COMSAT Institute of Information Technology Abbotabad, Abbotabad 22060, PakistanDepartment of Zoology, Kohat University of Science and Technology, Kohat, Pakistan; Corresponding author at: Department of Zoology, Kohat University of Science and Technology, Kohat, Pakistan.BRC, University of Karachi, Karachi, PakistanCentre of Excellence in Marine Biology, University of Karachi, Karachi, PakistanToxicology Laboratory, Department of Zoology, University of Karachi, Karachi, PakistanFaculty of Marine Science, Lasbela University of Agriculture, Water and Marine Sciences, Uthal, Balochistan, PakistanBee Research Chair, Department of Plant Protection, College of Food and Agriculture Sciences, King Saud University, Riyadh, Saudi ArabiaCedrus deodara (deodar) is practically used, as insect repellent, in the northern areas of Pakistan but no data available therefore this study was conducted to evaluate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (Tenebrio molitor), by feeding method. The aim of the study was to investigate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (Tenebrio molitor), by feeding method. All tested chemicals showed efficacy against the pests. The LC50 was determined by probit analysis and was found to be 3.41, 0.086 and 0.023% of larvae treated with deodar oil, Carbosulfan and Imidacloprid respectively The LC50 treated larvae were subjected to the evaluation of protein activity, qualitatively and quantitatively. The protein level in tested insects was enhanced when treated with Imidacloprid, Carbosulfan and deodar oil. The electrophoretic profile of treated insects showed more bands in insects treated with Cedrus deodara oil. This electrophoretic profile appeared in 4, 5, 7 and 8 bands for tested chemicals including control. Antifeedant activity was observed for C. deodara as larvae were deterred to feed on the food found in the container. Keywords: Tenebrio, Mealworms, Cedrus deodara, Imidacloprid, Carbosulfan, Protein level, Toxicityhttp://www.sciencedirect.com/science/article/pii/S1319562X17301547
collection DOAJ
language English
format Article
sources DOAJ
author Islam Dad Buneri
Masarrat Yousuf
Mohammad Attaullah
Saifullah Afridi
Syed Ishtiaq Anjum
Habibullah Rana
Naveed Ahmad
Muhammad Amin
Muhammad Tahir
Mohammad Javed Ansari
spellingShingle Islam Dad Buneri
Masarrat Yousuf
Mohammad Attaullah
Saifullah Afridi
Syed Ishtiaq Anjum
Habibullah Rana
Naveed Ahmad
Muhammad Amin
Muhammad Tahir
Mohammad Javed Ansari
A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)
Saudi Journal of Biological Sciences
author_facet Islam Dad Buneri
Masarrat Yousuf
Mohammad Attaullah
Saifullah Afridi
Syed Ishtiaq Anjum
Habibullah Rana
Naveed Ahmad
Muhammad Amin
Muhammad Tahir
Mohammad Javed Ansari
author_sort Islam Dad Buneri
title A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)
title_short A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)
title_full A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)
title_fullStr A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)
title_full_unstemmed A comparative toxic effect of Cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (Tenebrio molitor) (Coleoptera: Tenebrionidae)
title_sort comparative toxic effect of cedrus deodara oil on larval protein contents and its behavioral effect on larvae of mealworm beetle (tenebrio molitor) (coleoptera: tenebrionidae)
publisher Elsevier
series Saudi Journal of Biological Sciences
issn 1319-562X
publishDate 2019-02-01
description Cedrus deodara (deodar) is practically used, as insect repellent, in the northern areas of Pakistan but no data available therefore this study was conducted to evaluate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (Tenebrio molitor), by feeding method. The aim of the study was to investigate the effectiveness of deodar oil as an alternate of conventional insecticides against the larval pest stage of mealworm beetle (Tenebrio molitor), by feeding method. All tested chemicals showed efficacy against the pests. The LC50 was determined by probit analysis and was found to be 3.41, 0.086 and 0.023% of larvae treated with deodar oil, Carbosulfan and Imidacloprid respectively The LC50 treated larvae were subjected to the evaluation of protein activity, qualitatively and quantitatively. The protein level in tested insects was enhanced when treated with Imidacloprid, Carbosulfan and deodar oil. The electrophoretic profile of treated insects showed more bands in insects treated with Cedrus deodara oil. This electrophoretic profile appeared in 4, 5, 7 and 8 bands for tested chemicals including control. Antifeedant activity was observed for C. deodara as larvae were deterred to feed on the food found in the container. Keywords: Tenebrio, Mealworms, Cedrus deodara, Imidacloprid, Carbosulfan, Protein level, Toxicity
url http://www.sciencedirect.com/science/article/pii/S1319562X17301547
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