How to Address the Adjuvant Effects of Nanoparticles on the Immune System

• Main Authors: Alexia Feray, Natacha Szely, Eléonore Guillet, Marie Hullo, François-Xavier Legrand, Emilie Brun, Marc Pallardy, Armelle Biola-Vidamment
• Format: Article
• Language: English
• Published: MDPI AG 2020-02-01
• Series: Nanomaterials
• Subjects: amorphous silica nanoparticles; monocyte-derived dendritic cells; modcs; maturation; danger signal; dc:t-cell co-culture; in vitro models and methods; nanotoxicology
• Online Access: https://www.mdpi.com/2079-4991/10/3/425

As the nanotechnology market expands and the prevalence of allergic diseases keeps increasing, the knowledge gap on the capacity of nanomaterials to cause or exacerbate allergic outcomes needs more than ever to be filled. Engineered nanoparticles (NP) could have an adjuvant effect on the immune system as previously demonstrated for particulate air pollution. This effect would be the consequence of the recognition of NP as immune danger signals by dendritic cells (DCs). The aim of this work was to set up an <i>in vitro</i> method to functionally assess this effect using amorphous silica NP as a prototype. Most studies in this field are restricted to the evaluation of DCs maturation, generally of murine origin, through a limited phenotypic analysis. As it is essential to also consider the functional consequences of NP-induced DC altered phenotype on T-cells biology, we developed an allogeneic co-culture model of human monocyte-derived DCs (MoDCs) and CD4+ T-cells. We demonstrated that DC: T-cell ratios were a critical parameter to correctly measure the influence of NP danger signals through allogeneic co-culture. Moreover, to better visualize the effect of NP while minimizing the basal proliferation inherent to the model, we recommend testing three different ratios, preferably after five days of co-culture.