BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION

BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION

The optimization of the conditions of bacterial expression of recombinant tyrosyl-tRNA synthetase was conducted. The influence of the concentration of synthesis inductor of target protein at its final output and the best time of cultivation of bacterial culture before and after inductor adding was i...

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Bibliographic Details
Main Authors: Ю. Ю. Кондратюк, М. А. Бабарик, О. I. Корнелюк
Format: Article
Language:English
Published: Odessa I. I. Mechnikov National University 2009-12-01
Series:Mìkrobìologìâ ì Bìotehnologìâ
Subjects:
тирозил-трнк синтетаза
бактеріальна система експресії
оптимізація експресії
Online Access:http://mbt.onu.edu.ua/article/view/103197

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