BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION

BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION

The optimization of the conditions of bacterial expression of recombinant tyrosyl-tRNA synthetase was conducted. The influence of the concentration of synthesis inductor of target protein at its final output and the best time of cultivation of bacterial culture before and after inductor adding was i...

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Main Authors: Ю. Ю. Кондратюк, М. А. Бабарик, О. I. Корнелюк
Format: Article
Language:English
Published: Odessa I. I. Mechnikov National University 2009-12-01
Series:Mìkrobìologìâ ì Bìotehnologìâ
Subjects:
тирозил-трнк синтетаза
бактеріальна система експресії
оптимізація експресії
Online Access:http://mbt.onu.edu.ua/article/view/103197
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spelling doaj-b102d123f2b8438cbcdde0edc17e7adb2021-01-27T09:12:35ZengOdessa I. I. Mechnikov National University Mìkrobìologìâ ì Bìotehnologìâ2076-05582307-46632009-12-0104(8)61210.18524/2307-4663.2009.4(8).103197103197BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATIONЮ. Ю. Кондратюк0М. А. Бабарик1О. I. Корнелюк2Київський національний університет імені Тараса Шевченка; Iнститут молекулярної біології та генетики НАН УкраїниIнститут молекулярної біології та генетики НАН УкраїниIнститут молекулярної біології та генетики НАН УкраїниThe optimization of the conditions of bacterial expression of recombinant tyrosyl-tRNA synthetase was conducted. The influence of the concentration of synthesis inductor of target protein at its final output and the best time of cultivation of bacterial culture before and after inductor adding was investigated. The optimal conditions for culture E. coli BL21 (DE3) pLysE cultivation to achieve a high expression level of recombinant protein were proposed.http://mbt.onu.edu.ua/article/view/103197тирозил-трнк синтетазабактеріальна система експресіїоптимізація експресії
collection DOAJ
language English
format Article
sources DOAJ
author Ю. Ю. Кондратюк
М. А. Бабарик
О. I. Корнелюк
spellingShingle Ю. Ю. Кондратюк
М. А. Бабарик
О. I. Корнелюк
BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION
Mìkrobìologìâ ì Bìotehnologìâ
тирозил-трнк синтетаза
бактеріальна система експресії
оптимізація експресії
author_facet Ю. Ю. Кондратюк
М. А. Бабарик
О. I. Корнелюк
author_sort Ю. Ю. Кондратюк
title BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION
title_short BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION
title_full BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION
title_fullStr BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION
title_full_unstemmed BACTERIAL EXPRESSION OPTIMIZATION OF MAMMALIAN TYROSYL-TRNA SYNTHETASE ON STRAIN ESCHERICHIA COLI BL21 (DE3) PLYSE CULTIVATION
title_sort bacterial expression optimization of mammalian tyrosyl-trna synthetase on strain escherichia coli bl21 (de3) plyse cultivation
publisher Odessa I. I. Mechnikov National University
series Mìkrobìologìâ ì Bìotehnologìâ
issn 2076-0558
2307-4663
publishDate 2009-12-01
description The optimization of the conditions of bacterial expression of recombinant tyrosyl-tRNA synthetase was conducted. The influence of the concentration of synthesis inductor of target protein at its final output and the best time of cultivation of bacterial culture before and after inductor adding was investigated. The optimal conditions for culture E. coli BL21 (DE3) pLysE cultivation to achieve a high expression level of recombinant protein were proposed.
topic тирозил-трнк синтетаза
бактеріальна система експресії
оптимізація експресії
url http://mbt.onu.edu.ua/article/view/103197
work_keys_str_mv AT ûûkondratûk bacterialexpressionoptimizationofmammaliantyrosyltrnasynthetaseonstrainescherichiacolibl21de3plysecultivation
AT mababarik bacterialexpressionoptimizationofmammaliantyrosyltrnasynthetaseonstrainescherichiacolibl21de3plysecultivation
AT oikornelûk bacterialexpressionoptimizationofmammaliantyrosyltrnasynthetaseonstrainescherichiacolibl21de3plysecultivation
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