A Modified Protocol for Bisulfite Genomic Sequencing of Difficult Samples

<p>Abstract</p> <p>The bisulfite genomic sequencing protocol is a widely used method for analyzing DNA methylation. It relies on the deamination of unmethylated cytosine residues to uracil; however, its high rates of DNA degradation and incomplete cytosine to uracil conversion ofte...

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Bibliographic Details
Main Authors: Pappas Jane J, Toulouse André, Bradley WEC
Format: Article
Language:English
Published: BMC 2009-06-01
Series:Biological Procedures Online
Subjects:
Online Access:http://www.biologicalproceduresonline.com/content/11/1/9010