Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies
Although several genes are known to be deregulated in urinary bladder cancer (UBC), the list of candidate prognostic markers has expanded due to the advance of high-throughput methodologies, but they do not always accord from study to study. We aimed to detect global gene co-expressional profiles am...
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doaj-b0ebdaf3e251411aa7994d02171431002021-02-19T00:00:10ZengMDPI AGApplied Sciences2076-34172021-02-01111785178510.3390/app11041785Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput MethodologiesGeorge I. Lambrou0Kleanthis Vichos1Dimitrios Koutsouris2Apostolos Zaravinos3Choremeio Research Laboratory, First Department of Pediatrics, National and Kapodistrian University of Athens, 15127 Athens, GreeceBiomedical Engineering Laboratory, School of Electrical and Computer Engineering, National Technical University of Athens, 15780 Athens, GreeceBiomedical Engineering Laboratory, School of Electrical and Computer Engineering, National Technical University of Athens, 15780 Athens, GreeceDepartment of Basic Medical Sciences, College of Medicine, Member of QU Health, Qatar University, Doha 2713, QatarAlthough several genes are known to be deregulated in urinary bladder cancer (UBC), the list of candidate prognostic markers has expanded due to the advance of high-throughput methodologies, but they do not always accord from study to study. We aimed to detect global gene co-expressional profiles among a high number of UBC tumors. We mined gene expression data from 5 microarray datasets from GEO, containing 131 UBC and 15 normal samples. Data were analyzed using unsupervised classification algorithms. The application of clustering algorithms resulted in the isolation of 6 down-regulated genes (<i>TMP2, ACTC1, TAGLN, MFAP4, SPARCL1,</i> and <i>GLP1R</i>), which were mainly implicated in the proteasome, base excision repair, and DNA replication functions. We also detected 6 up-regulated genes (<i>CDC20, KRT14, APOBEC3B, MCM5, STMN,</i> and <i>YWHAB</i>) mainly involved in cancer pathways. We identified lists of drugs that could potentially associate with the Differentially Expressed Genes (DEGs), including Vardenafil, Pyridone 6, and Manganese (co-upregulated genes) or 1D-myo-inositol 1,4,5-triphosphate (co-down regulated genes). We propose 12 novel candidate markers for UBC, as well as potential drugs, shedding more light on the underlying cause of the development and progression of the disease.https://www.mdpi.com/2076-3417/11/4/1785urinary bladder cancermicroarraycommon gene expressionunsupervised machine learning algorithms |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
George I. Lambrou Kleanthis Vichos Dimitrios Koutsouris Apostolos Zaravinos |
spellingShingle |
George I. Lambrou Kleanthis Vichos Dimitrios Koutsouris Apostolos Zaravinos Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies Applied Sciences urinary bladder cancer microarray common gene expression unsupervised machine learning algorithms |
author_facet |
George I. Lambrou Kleanthis Vichos Dimitrios Koutsouris Apostolos Zaravinos |
author_sort |
George I. Lambrou |
title |
Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies |
title_short |
Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies |
title_full |
Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies |
title_fullStr |
Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies |
title_full_unstemmed |
Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies |
title_sort |
identification of co-deregulated genes in urinary bladder cancer using high-throughput methodologies |
publisher |
MDPI AG |
series |
Applied Sciences |
issn |
2076-3417 |
publishDate |
2021-02-01 |
description |
Although several genes are known to be deregulated in urinary bladder cancer (UBC), the list of candidate prognostic markers has expanded due to the advance of high-throughput methodologies, but they do not always accord from study to study. We aimed to detect global gene co-expressional profiles among a high number of UBC tumors. We mined gene expression data from 5 microarray datasets from GEO, containing 131 UBC and 15 normal samples. Data were analyzed using unsupervised classification algorithms. The application of clustering algorithms resulted in the isolation of 6 down-regulated genes (<i>TMP2, ACTC1, TAGLN, MFAP4, SPARCL1,</i> and <i>GLP1R</i>), which were mainly implicated in the proteasome, base excision repair, and DNA replication functions. We also detected 6 up-regulated genes (<i>CDC20, KRT14, APOBEC3B, MCM5, STMN,</i> and <i>YWHAB</i>) mainly involved in cancer pathways. We identified lists of drugs that could potentially associate with the Differentially Expressed Genes (DEGs), including Vardenafil, Pyridone 6, and Manganese (co-upregulated genes) or 1D-myo-inositol 1,4,5-triphosphate (co-down regulated genes). We propose 12 novel candidate markers for UBC, as well as potential drugs, shedding more light on the underlying cause of the development and progression of the disease. |
topic |
urinary bladder cancer microarray common gene expression unsupervised machine learning algorithms |
url |
https://www.mdpi.com/2076-3417/11/4/1785 |
work_keys_str_mv |
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