Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies

Although several genes are known to be deregulated in urinary bladder cancer (UBC), the list of candidate prognostic markers has expanded due to the advance of high-throughput methodologies, but they do not always accord from study to study. We aimed to detect global gene co-expressional profiles am...

Full description

Bibliographic Details
Main Authors: George I. Lambrou, Kleanthis Vichos, Dimitrios Koutsouris, Apostolos Zaravinos
Format: Article
Language:English
Published: MDPI AG 2021-02-01
Series:Applied Sciences
Subjects:
Online Access:https://www.mdpi.com/2076-3417/11/4/1785
id doaj-b0ebdaf3e251411aa7994d0217143100
record_format Article
spelling doaj-b0ebdaf3e251411aa7994d02171431002021-02-19T00:00:10ZengMDPI AGApplied Sciences2076-34172021-02-01111785178510.3390/app11041785Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput MethodologiesGeorge I. Lambrou0Kleanthis Vichos1Dimitrios Koutsouris2Apostolos Zaravinos3Choremeio Research Laboratory, First Department of Pediatrics, National and Kapodistrian University of Athens, 15127 Athens, GreeceBiomedical Engineering Laboratory, School of Electrical and Computer Engineering, National Technical University of Athens, 15780 Athens, GreeceBiomedical Engineering Laboratory, School of Electrical and Computer Engineering, National Technical University of Athens, 15780 Athens, GreeceDepartment of Basic Medical Sciences, College of Medicine, Member of QU Health, Qatar University, Doha 2713, QatarAlthough several genes are known to be deregulated in urinary bladder cancer (UBC), the list of candidate prognostic markers has expanded due to the advance of high-throughput methodologies, but they do not always accord from study to study. We aimed to detect global gene co-expressional profiles among a high number of UBC tumors. We mined gene expression data from 5 microarray datasets from GEO, containing 131 UBC and 15 normal samples. Data were analyzed using unsupervised classification algorithms. The application of clustering algorithms resulted in the isolation of 6 down-regulated genes (<i>TMP2, ACTC1, TAGLN, MFAP4, SPARCL1,</i> and <i>GLP1R</i>), which were mainly implicated in the proteasome, base excision repair, and DNA replication functions. We also detected 6 up-regulated genes (<i>CDC20, KRT14, APOBEC3B, MCM5, STMN,</i> and <i>YWHAB</i>) mainly involved in cancer pathways. We identified lists of drugs that could potentially associate with the Differentially Expressed Genes (DEGs), including Vardenafil, Pyridone 6, and Manganese (co-upregulated genes) or 1D-myo-inositol 1,4,5-triphosphate (co-down regulated genes). We propose 12 novel candidate markers for UBC, as well as potential drugs, shedding more light on the underlying cause of the development and progression of the disease.https://www.mdpi.com/2076-3417/11/4/1785urinary bladder cancermicroarraycommon gene expressionunsupervised machine learning algorithms
collection DOAJ
language English
format Article
sources DOAJ
author George I. Lambrou
Kleanthis Vichos
Dimitrios Koutsouris
Apostolos Zaravinos
spellingShingle George I. Lambrou
Kleanthis Vichos
Dimitrios Koutsouris
Apostolos Zaravinos
Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies
Applied Sciences
urinary bladder cancer
microarray
common gene expression
unsupervised machine learning algorithms
author_facet George I. Lambrou
Kleanthis Vichos
Dimitrios Koutsouris
Apostolos Zaravinos
author_sort George I. Lambrou
title Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies
title_short Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies
title_full Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies
title_fullStr Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies
title_full_unstemmed Identification of Co-Deregulated Genes in Urinary Bladder Cancer Using High-Throughput Methodologies
title_sort identification of co-deregulated genes in urinary bladder cancer using high-throughput methodologies
publisher MDPI AG
series Applied Sciences
issn 2076-3417
publishDate 2021-02-01
description Although several genes are known to be deregulated in urinary bladder cancer (UBC), the list of candidate prognostic markers has expanded due to the advance of high-throughput methodologies, but they do not always accord from study to study. We aimed to detect global gene co-expressional profiles among a high number of UBC tumors. We mined gene expression data from 5 microarray datasets from GEO, containing 131 UBC and 15 normal samples. Data were analyzed using unsupervised classification algorithms. The application of clustering algorithms resulted in the isolation of 6 down-regulated genes (<i>TMP2, ACTC1, TAGLN, MFAP4, SPARCL1,</i> and <i>GLP1R</i>), which were mainly implicated in the proteasome, base excision repair, and DNA replication functions. We also detected 6 up-regulated genes (<i>CDC20, KRT14, APOBEC3B, MCM5, STMN,</i> and <i>YWHAB</i>) mainly involved in cancer pathways. We identified lists of drugs that could potentially associate with the Differentially Expressed Genes (DEGs), including Vardenafil, Pyridone 6, and Manganese (co-upregulated genes) or 1D-myo-inositol 1,4,5-triphosphate (co-down regulated genes). We propose 12 novel candidate markers for UBC, as well as potential drugs, shedding more light on the underlying cause of the development and progression of the disease.
topic urinary bladder cancer
microarray
common gene expression
unsupervised machine learning algorithms
url https://www.mdpi.com/2076-3417/11/4/1785
work_keys_str_mv AT georgeilambrou identificationofcoderegulatedgenesinurinarybladdercancerusinghighthroughputmethodologies
AT kleanthisvichos identificationofcoderegulatedgenesinurinarybladdercancerusinghighthroughputmethodologies
AT dimitrioskoutsouris identificationofcoderegulatedgenesinurinarybladdercancerusinghighthroughputmethodologies
AT apostoloszaravinos identificationofcoderegulatedgenesinurinarybladdercancerusinghighthroughputmethodologies
_version_ 1724262097654644736