APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES

APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES

Abstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) ar...

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Main Authors: M. A. Plotnikova, S. A. Klotchenko, V. V. Egorov, K. P. Nikulenkov, A. V. Vasin, O. I. Kisselev
Format: Article
Language:Russian
Published: SPb RAACI 2014-07-01
Series:Medicinskaâ Immunologiâ
Subjects:
cytokines
antibodies
microarray
immunological analysis
Online Access:https://www.mimmun.ru/mimmun/article/view/378
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spelling doaj-b0c4e1a20bfc4d05bc7aa47d164237922021-07-29T09:02:24ZrusSPb RAACIMedicinskaâ Immunologiâ1563-06252313-741X2014-07-01144-541541810.15789/1563-0625-2012-4-5-415-418375APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINESM. A. Plotnikova0S. A. Klotchenko1V. V. Egorov2K. P. Nikulenkov3A. V. Vasin4O. I. Kisselev5ФГБУ «Научно-исследовательский институт гриппа» Минздравсоцразвития РФ, Санкт-ПетербургФГБУ «Научно-исследовательский институт гриппа» Минздравсоцразвития РФ, Санкт-ПетербургФГБУ «Научно-исследовательский институт гриппа» Минздравсоцразвития РФ, Санкт-ПетербургФГБУ «Научно-исследовательский институт гриппа» Минздравсоцразвития РФ, Санкт-ПетербургФГБУ «Научно-исследовательский институт гриппа» Минздравсоцразвития РФ, Санкт-ПетербургФГБУ «Научно-исследовательский институт гриппа» Минздравсоцразвития РФ, Санкт-ПетербургAbstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) are demonstrated with human IL-2, IL-4, IL-10, and TNF assays. This diagnostic technique may be further used both for research applications and in clinical trials.https://www.mimmun.ru/mimmun/article/view/378cytokinesantibodiesmicroarrayimmunological analysis
collection DOAJ
language Russian
format Article
sources DOAJ
author M. A. Plotnikova
S. A. Klotchenko
V. V. Egorov
K. P. Nikulenkov
A. V. Vasin
O. I. Kisselev
spellingShingle M. A. Plotnikova
S. A. Klotchenko
V. V. Egorov
K. P. Nikulenkov
A. V. Vasin
O. I. Kisselev
APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
Medicinskaâ Immunologiâ
cytokines
antibodies
microarray
immunological analysis
author_facet M. A. Plotnikova
S. A. Klotchenko
V. V. Egorov
K. P. Nikulenkov
A. V. Vasin
O. I. Kisselev
author_sort M. A. Plotnikova
title APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_short APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_full APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_fullStr APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_full_unstemmed APPLICATION OF SOLID-PHASE, ENZYME-LINKED IMMUNOASSAYS IN MICROARRAY FORMATE FOR QUANTITATIVE DETERMINATION OF CYTOKINES
title_sort application of solid-phase, enzyme-linked immunoassays in microarray formate for quantitative determination of cytokines
publisher SPb RAACI
series Medicinskaâ Immunologiâ
issn 1563-0625
2313-741X
publishDate 2014-07-01
description Abstract. Present work describes a multiplex “sandvich” approach to enzyme-linked, solid-phase immunosorbent assay designed in microarray formate, aiming for quantitative evaluation of individual profile of cytokine expression. Its high specificity, throughput and sensitivity (30 to 20,000 pg/mL) are demonstrated with human IL-2, IL-4, IL-10, and TNF assays. This diagnostic technique may be further used both for research applications and in clinical trials.
topic cytokines
antibodies
microarray
immunological analysis
url https://www.mimmun.ru/mimmun/article/view/378
work_keys_str_mv AT maplotnikova applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines
AT saklotchenko applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines
AT vvegorov applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines
AT kpnikulenkov applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines
AT avvasin applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines
AT oikisselev applicationofsolidphaseenzymelinkedimmunoassaysinmicroarrayformateforquantitativedeterminationofcytokines
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