Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic Strokes
Introduction: To evaluate Low-Molecular-Weight (LMW) DNA as a possible prognostic biomarker in acute ischemic and hemorrhagic stroke. Methods: LMW DNA samples were isolated from plasma and cerebrospinal fluid by phenol deproteinization, analyzed by gradient polyacrylamide electrophoresis and quantif...
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Iran University of Medical Sciences
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doaj-b0bd38a2130c42489241c7b78991beaa2021-02-20T09:49:03ZengIran University of Medical SciencesBasic and Clinical Neuroscience2008-126X2228-74422020-11-01116805810Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic StrokesIrina Vasilyeva0Vladimir Bespalov1Ancha Baranova2Igor Voznyuk3Denis Baranenko4 Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N.Petrov National Medical Research Center of Oncology, St.-Petersburg, Russia. Laboratory of Cancer Chemoprevention and Oncopharmacology, N.N.Petrov National Medical Research Center of Oncology, St.-Petersburg, Russia. Department of George, School of Systemic Biology, George Mason University, Fairfax, VA, USA. Department of Acute Cerebrovascular Pathology and Emergency Neurology, Saint-Petersburg I.I. Dzhanelidze Research Institute for Emergency Medicine, St.-Petersburg, Russia. Department of Acute Cerebrovascular Pathology and Emergency Neurology, Saint-Petersburg I.I. Dzhanelidze Research Institute for Emergency Medicine, St.-Petersburg, Russia. Introduction: To evaluate Low-Molecular-Weight (LMW) DNA as a possible prognostic biomarker in acute ischemic and hemorrhagic stroke. Methods: LMW DNA samples were isolated from plasma and cerebrospinal fluid by phenol deproteinization, analyzed by gradient polyacrylamide electrophoresis and quantified by spectrophotometry. Results: Two common types of stroke, i.e. ischemic and hemorrhagic, differ by the temporal dynamics of cell-free DNA (cfDNA) accumulation. In hemorrhagic stroke, an initial increase in LMW DNA levels, most likely reflects an extent of the tissue damage, while in ischemic patients, the LMW DNA levels increase in parallel with the damage caused by hypoxia and subsequent compensatory reperfusion. Conclusion: These time-course data specify optimal assessment windows with maximum differentiating power for stroke outcomes: 24-48 hours post-event for ischemic stroke, and as close as possible to the moment of hospital admission for hemorrhagic stroke. These data also indicate the role of apoptosis in the formation of ischemic focus.http://bcn.iums.ac.ir/article-1-1386-en.htmlacute cerebrovascular accidentcerebral ischemiahemorrhagic strokecell-free dnaapoptosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Irina Vasilyeva Vladimir Bespalov Ancha Baranova Igor Voznyuk Denis Baranenko |
spellingShingle |
Irina Vasilyeva Vladimir Bespalov Ancha Baranova Igor Voznyuk Denis Baranenko Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic Strokes Basic and Clinical Neuroscience acute cerebrovascular accident cerebral ischemia hemorrhagic stroke cell-free dna apoptosis |
author_facet |
Irina Vasilyeva Vladimir Bespalov Ancha Baranova Igor Voznyuk Denis Baranenko |
author_sort |
Irina Vasilyeva |
title |
Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic Strokes |
title_short |
Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic Strokes |
title_full |
Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic Strokes |
title_fullStr |
Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic Strokes |
title_full_unstemmed |
Differential Dynamics of the Levels of Low Molecular Weight DNA Fragments in the Plasma of Patients With Ischemic and Hemorrhagic Strokes |
title_sort |
differential dynamics of the levels of low molecular weight dna fragments in the plasma of patients with ischemic and hemorrhagic strokes |
publisher |
Iran University of Medical Sciences |
series |
Basic and Clinical Neuroscience |
issn |
2008-126X 2228-7442 |
publishDate |
2020-11-01 |
description |
Introduction: To evaluate Low-Molecular-Weight (LMW) DNA as a possible prognostic biomarker in acute ischemic and hemorrhagic stroke.
Methods: LMW DNA samples were isolated from plasma and cerebrospinal fluid by phenol deproteinization, analyzed by gradient polyacrylamide electrophoresis and quantified by spectrophotometry.
Results: Two common types of stroke, i.e. ischemic and hemorrhagic, differ by the temporal dynamics of cell-free DNA (cfDNA) accumulation. In hemorrhagic stroke, an initial increase in LMW DNA levels, most likely reflects an extent of the tissue damage, while in ischemic patients, the LMW DNA levels increase in parallel with the damage caused by hypoxia and subsequent compensatory reperfusion.
Conclusion: These time-course data specify optimal assessment windows with maximum differentiating power for stroke outcomes: 24-48 hours post-event for ischemic stroke, and as close as possible to the moment of hospital admission for hemorrhagic stroke. These data also indicate the role of apoptosis in the formation of ischemic focus. |
topic |
acute cerebrovascular accident cerebral ischemia hemorrhagic stroke cell-free dna apoptosis |
url |
http://bcn.iums.ac.ir/article-1-1386-en.html |
work_keys_str_mv |
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