Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation

Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation

Multiple myeloma (MM) is a hematological cancer characterized by an uncontrolled proliferation of antibody-secreting plasma cells within the bone marrow. Currently, cell therapy such as chimeric antigen receptor T-cell (CAR-T) based on induced pluripotent stem cells (iPSCs) has received attention fo...

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Main Authors: Seung-Jin Lee, Ji-Hea Kim, Ka-Won Kang, Byung-Hyun Lee, Byung-Soo Kim
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120303317
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spelling doaj-b0b09b4ceb224ba68dd8a2fcc960c8202020-12-19T05:04:53ZengElsevierStem Cell Research1873-50612020-12-0149102030Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocationSeung-Jin Lee0Ji-Hea Kim1Ka-Won Kang2Byung-Hyun Lee3Byung-Soo Kim4Institute of Stem Cell Research, Korea University College of Medicine, Seoul, South Korea; BK21PLUS Graduate Program of Korea University College of Medicine, Seoul, South KoreaInstitute of Stem Cell Research, Korea University College of Medicine, Seoul, South Korea; BK21PLUS Graduate Program of Korea University College of Medicine, Seoul, South KoreaDepartment of Internal Medicine, Korea University Anam Medical Center, Seoul, South KoreaDepartment of Internal Medicine, Korea University Anam Medical Center, Seoul, South KoreaInstitute of Stem Cell Research, Korea University College of Medicine, Seoul, South Korea; BK21PLUS Graduate Program of Korea University College of Medicine, Seoul, South Korea; Department of Internal Medicine, Korea University Anam Medical Center, Seoul, South Korea; Corresponding author at: Institute of Stem Cell Research, Korea University College of Medicine, Seoul, South Korea.Multiple myeloma (MM) is a hematological cancer characterized by an uncontrolled proliferation of antibody-secreting plasma cells within the bone marrow. Currently, cell therapy such as chimeric antigen receptor T-cell (CAR-T) based on induced pluripotent stem cells (iPSCs) has received attention for treating MM. However, the generation of iPSCs from MM patients appears to be very rarely reported. Here we generated an iPSC line from CD34+ bone marrow cells of a patient with MM using human placenta-derived cell conditioned medium (hPCCM), offering a relatively high efficiency in humanized conditions. This iPSC line might be a useful model for research on MM.http://www.sciencedirect.com/science/article/pii/S1873506120303317
collection DOAJ
language English
format Article
sources DOAJ
author Seung-Jin Lee
Ji-Hea Kim
Ka-Won Kang
Byung-Hyun Lee
Byung-Soo Kim
spellingShingle Seung-Jin Lee
Ji-Hea Kim
Ka-Won Kang
Byung-Hyun Lee
Byung-Soo Kim
Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation
Stem Cell Research
author_facet Seung-Jin Lee
Ji-Hea Kim
Ka-Won Kang
Byung-Hyun Lee
Byung-Soo Kim
author_sort Seung-Jin Lee
title Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation
title_short Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation
title_full Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation
title_fullStr Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation
title_full_unstemmed Generation of normal induced pluripotent stem cell line KUMCi002-A from bone marrow CD34+ cells of patient with multiple myeloma disease having 13q deletion and IGH translocation
title_sort generation of normal induced pluripotent stem cell line kumci002-a from bone marrow cd34+ cells of patient with multiple myeloma disease having 13q deletion and igh translocation
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-12-01
description Multiple myeloma (MM) is a hematological cancer characterized by an uncontrolled proliferation of antibody-secreting plasma cells within the bone marrow. Currently, cell therapy such as chimeric antigen receptor T-cell (CAR-T) based on induced pluripotent stem cells (iPSCs) has received attention for treating MM. However, the generation of iPSCs from MM patients appears to be very rarely reported. Here we generated an iPSC line from CD34+ bone marrow cells of a patient with MM using human placenta-derived cell conditioned medium (hPCCM), offering a relatively high efficiency in humanized conditions. This iPSC line might be a useful model for research on MM.
url http://www.sciencedirect.com/science/article/pii/S1873506120303317
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