Ultrastructural study of chloroplasts isolated by various fractionation methods

Ultrastructural study of chloroplasts isolated by various fractionation methods

The chloroplasts of kohlrabi and barley leaves isolated in conventional media and examined in electron microscope showed considerable impairements of their structure. The damages were not eliminated if the centrifugation number was limited and the tonicity of the medium was reduced gradually through...

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Main Authors: J. Legocka, A. Woźny, A. Szweykowska
Format: Article
Language:English
Published: Polish Botanical Society 2015-01-01
Series:Acta Societatis Botanicorum Poloniae
Online Access:https://pbsociety.org.pl/journals/index.php/asbp/article/view/5039
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spelling doaj-b08642f6cd724d5e8b901eabea4b316d2020-11-25T03:24:48ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802015-01-0144344344810.5586/asbp.1975.0404241Ultrastructural study of chloroplasts isolated by various fractionation methodsJ. Legocka0A. Woźny1A. Szweykowska2Adam Mickiewicz UniversityAdam Mickiewicz UniversityAdam Mickiewicz UniversityThe chloroplasts of kohlrabi and barley leaves isolated in conventional media and examined in electron microscope showed considerable impairements of their structure. The damages were not eliminated if the centrifugation number was limited and the tonicity of the medium was reduced gradually throughout the fixation and dehydration procedure. A good yield (about 90%) of structurally intact kohlrabi chloroplasts was obtained when they were isolated from leaves pre-fixed and homogenized in medium containing glutaraldehyde.https://pbsociety.org.pl/journals/index.php/asbp/article/view/5039
collection DOAJ
language English
format Article
sources DOAJ
author J. Legocka
A. Woźny
A. Szweykowska
spellingShingle J. Legocka
A. Woźny
A. Szweykowska
Ultrastructural study of chloroplasts isolated by various fractionation methods
Acta Societatis Botanicorum Poloniae
author_facet J. Legocka
A. Woźny
A. Szweykowska
author_sort J. Legocka
title Ultrastructural study of chloroplasts isolated by various fractionation methods
title_short Ultrastructural study of chloroplasts isolated by various fractionation methods
title_full Ultrastructural study of chloroplasts isolated by various fractionation methods
title_fullStr Ultrastructural study of chloroplasts isolated by various fractionation methods
title_full_unstemmed Ultrastructural study of chloroplasts isolated by various fractionation methods
title_sort ultrastructural study of chloroplasts isolated by various fractionation methods
publisher Polish Botanical Society
series Acta Societatis Botanicorum Poloniae
issn 2083-9480
publishDate 2015-01-01
description The chloroplasts of kohlrabi and barley leaves isolated in conventional media and examined in electron microscope showed considerable impairements of their structure. The damages were not eliminated if the centrifugation number was limited and the tonicity of the medium was reduced gradually throughout the fixation and dehydration procedure. A good yield (about 90%) of structurally intact kohlrabi chloroplasts was obtained when they were isolated from leaves pre-fixed and homogenized in medium containing glutaraldehyde.
url https://pbsociety.org.pl/journals/index.php/asbp/article/view/5039
work_keys_str_mv AT jlegocka ultrastructuralstudyofchloroplastsisolatedbyvariousfractionationmethods
AT awozny ultrastructuralstudyofchloroplastsisolatedbyvariousfractionationmethods
AT aszweykowska ultrastructuralstudyofchloroplastsisolatedbyvariousfractionationmethods
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