Proteomic analysis of fertilized egg white during early incubation

Proteomic analysis of fertilized egg white during early incubation

Proteomic analysis of egg white proteins was performed to elucidate their metabolic fates during first days of embryo development using 2-DE coupled with a LC–MS/MS. A total of 91 protein spots were analyzed, representing 37 proteins belonging to ‘Gallus gallus’, of 19 proteins were detected in egg...

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Bibliographic Details
Main Authors: Jiapei Wang, Jianping Wu
Format: Article
Language:English
Published: Elsevier 2014-03-01
Series:EuPA Open Proteomics
Subjects:
Fertilized egg whites
2-DE
Proteomics
Incubation
Quantification
Embryo
Online Access:http://www.sciencedirect.com/science/article/pii/S2212968513000081
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spelling doaj-b05e4bf08cd44b70aa65635236cc122e2020-11-24T22:35:01ZengElsevierEuPA Open Proteomics2212-96852014-03-012C385910.1016/j.euprot.2013.11.001Proteomic analysis of fertilized egg white during early incubationJiapei WangJianping WuProteomic analysis of egg white proteins was performed to elucidate their metabolic fates during first days of embryo development using 2-DE coupled with a LC–MS/MS. A total of 91 protein spots were analyzed, representing 37 proteins belonging to ‘Gallus gallus’, of 19 proteins were detected in egg whites for the first time, such as lipoproteins, vitellogenin and zona pellucida C protein. All ovomucoid spots with one exception were significantly (P < 0.05) increased. Marker protein and one flavoprotein spot were significantly increased while hemopexin, serum albumin precursor, Ex-FABP precursor and Galline Ex-FABP were significantly decreased.http://www.sciencedirect.com/science/article/pii/S2212968513000081Fertilized egg whites2-DEProteomicsIncubationQuantificationEmbryo
collection DOAJ
language English
format Article
sources DOAJ
author Jiapei Wang
Jianping Wu
spellingShingle Jiapei Wang
Jianping Wu
Proteomic analysis of fertilized egg white during early incubation
EuPA Open Proteomics
Fertilized egg whites
2-DE
Proteomics
Incubation
Quantification
Embryo
author_facet Jiapei Wang
Jianping Wu
author_sort Jiapei Wang
title Proteomic analysis of fertilized egg white during early incubation
title_short Proteomic analysis of fertilized egg white during early incubation
title_full Proteomic analysis of fertilized egg white during early incubation
title_fullStr Proteomic analysis of fertilized egg white during early incubation
title_full_unstemmed Proteomic analysis of fertilized egg white during early incubation
title_sort proteomic analysis of fertilized egg white during early incubation
publisher Elsevier
series EuPA Open Proteomics
issn 2212-9685
publishDate 2014-03-01
description Proteomic analysis of egg white proteins was performed to elucidate their metabolic fates during first days of embryo development using 2-DE coupled with a LC–MS/MS. A total of 91 protein spots were analyzed, representing 37 proteins belonging to ‘Gallus gallus’, of 19 proteins were detected in egg whites for the first time, such as lipoproteins, vitellogenin and zona pellucida C protein. All ovomucoid spots with one exception were significantly (P < 0.05) increased. Marker protein and one flavoprotein spot were significantly increased while hemopexin, serum albumin precursor, Ex-FABP precursor and Galline Ex-FABP were significantly decreased.
topic Fertilized egg whites
2-DE
Proteomics
Incubation
Quantification
Embryo
url http://www.sciencedirect.com/science/article/pii/S2212968513000081
work_keys_str_mv AT jiapeiwang proteomicanalysisoffertilizedeggwhiteduringearlyincubation
AT jianpingwu proteomicanalysisoffertilizedeggwhiteduringearlyincubation
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