1,2-3H-Cholesterol as a tracer in studies of human cholesterol metabolism

1,2-3H-Cholesterol as a tracer in studies of human cholesterol metabolism

1,2-3H-Cholesterol was fed to a subject together with 4-14C-cholesterol at a known 3H/14C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile.Isotopic fractionation was seen during thin-layer chromatographic isolation of...

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Bibliographic Details
Main Authors: Peter D.S. Wood, Daphne Myers, Yuen-Ling Lee, Ryuzo Shioda, Laurance W. Kinsell
Format: Article
Language:English
Published: Elsevier 1967-07-01
Series:Journal of Lipid Research
Subjects:
cholesterol
1,2-3H-cholesterol
4-14C-cholesterol
thin-layer chromatography
isotope effect
plasma
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520395730
Description
Summary:1,2-3H-Cholesterol was fed to a subject together with 4-14C-cholesterol at a known 3H/14C ratio. The ratio was satisfactorily preserved in cholesterol recovered from plasma, red cells, and bile, and in bile acids of bile.Isotopic fractionation was seen during thin-layer chromatographic isolation of cholesterol. In work with 1,2-3H-cholesterol or its metabolites care should be taken to recover chromatographic bands in their entirety.
ISSN:0022-2275

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