Profile of serum alkaline phosphatase after inoculation of mononuclear cells and bone morphogenetic protein in the repair of osteochondral defects in rabbits

• Main Authors: Luiz Augusto de Souza, Benito Juarez Nunes Alves de Oliveira, Duvaldo Eurides, Ednaldo Carvalho Guimarães, Luiz Antônio Franco da Silva, Lorena Borges Alves, Ana Flávia Delben Pereira Arruda, Taís Andrade Dias
• Format: Article
• Language: English
• Published: Universidade Federal de Santa Catarina 2011-12-01
• Series: Biotemas
• Subjects: Bone marker; Fracture healing; Stem cells
• Online Access: http://www.biotemas.ufsc.br/volumes/pdf/volume244/103a109.pdf

In this study, serum alkaline phosphatase activity was measured in response to the repair of osteochondral defects in twenty-four New Zealand rabbits. The animals were divided into three groups: a control (GC), those treated with bone marrow mononuclear cells (GCM) and those that received mononuclear cells with autologous bone morphogenetic protein (BMP + GCM). After exposing the trochlear groove of the left stifle joint, a wedge-shaped segment was removed. Later, the defect was filled with an osteochondral autograft preserved in 98% glycerin. For the GC group, only the bone graft was performed. For the GCM, in addition to the graft, 2x106 seed mononuclear cells were implanted. For the GCM + BMP, the same number of cells, associated with 1μg of bone morphogenetic protein, were intraarticularly administered. The osteoblastic response was measured by analyzing the serum alkaline phosphatase on day 0 (preoperative) 3, 15, 30, and 45 after surgery, and by radiographic examinations. Analysis of variance in randomized blocks, factorial and Tukey’s test (p = 0.05) were made. The overall mean GCM was superior to the other groups and the highest rates were among the 15th and 45th days postoperatively. The discrepancy in values between individuals of the same group casts doubts on the veracity of the test.