Direct RAPD evaluation of bacteria without conventional DNA extraction

Direct RAPD evaluation of bacteria without conventional DNA extraction

The present work reports successful DNA amplification of Pantoea agglomerans and Bacillus pumilus through Random Amplified Polymorphic DNA (RAPD). For this, template DNA was obtained without conventional DNA extraction. The procedure was as follows: cultures grown for 20 hours in 5 mL LB medium were...

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Bibliographic Details
Main Authors:	Welington Luiz Araújo, Derlene Attili de Angellis, João Lúcio Azevedo
Format:	Article
Language:	English
Published:	Instituto de Tecnologia do Paraná (Tecpar) 2004-07-01
Series:	Brazilian Archives of Biology and Technology
Subjects:	Bacteria boiling method DNA extraction RAPD
Online Access:	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300006

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