BART-Seq: cost-effective massively parallelized targeted sequencing for genomics, transcriptomics, and single-cell analysis

BART-Seq: cost-effective massively parallelized targeted sequencing for genomics, transcriptomics, and single-cell analysis

Abstract We describe a highly sensitive, quantitative, and inexpensive technique for targeted sequencing of transcript cohorts or genomic regions from thousands of bulk samples or single cells in parallel. Multiplexing is based on a simple method that produces extensive matrices of diverse DNA barco...

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Bibliographic Details
Main Authors: Fatma Uzbas, Florian Opperer, Can Sönmezer, Dmitry Shaposhnikov, Steffen Sass, Christian Krendl, Philipp Angerer, Fabian J. Theis, Nikola S. Mueller, Micha Drukker
Format: Article
Language:English
Published: BMC 2019-08-01
Series:Genome Biology
Subjects:
Barcoding
Single-cell RNA sequencing
Targeted transcriptomics
High-throughput screening
Human pluripotent stem cells
Multiplex PCR
Online Access:http://link.springer.com/article/10.1186/s13059-019-1748-6

Internet

http://link.springer.com/article/10.1186/s13059-019-1748-6

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