Results of Tests of Disinfecting Materials in Regard to Melioidosis and Glanders Agents

• Main Authors: D. N. Luchinin, E. V. Molchanova, K. A. Rotov, A. O. Negodenko, D. V. Viktorov
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2020-01-01
• Series: Проблемы особо опасных инфекций
• Subjects: disinfectant; test strain; disinfection efficiency; <i>b. mallei</i>; <i>b. pseudomallei</i>
• Online Access: https://journal.microbe.ru/jour/article/view/1224
• ISSN: 0370-1069; 2658-719X

Objective of the work was to conduct tests of disinfectants against pathogens of melioidosis and glanders. Materials and methods. 10 strains of the causative agent of melioidosis, 4 strains of the glanders pathogen, and 5 strains of B. thailandensis were studied. All of them possessed biochemical, morphological, tinctorial, cultural and enzymatic properties typical of the respective species. The following disinfectants were used in the study: alkyldimethylbenzylammonium chloride (Sigma-Aldrich, USA), glutaraldehyde, chloramine B, hydrogen peroxide medical, MD-1 (Medical Disinfection LLC, Russia), CAT-18 (LLC Satellite, Russia), SAT-19 (Satellite LLC, Russia), ODS-15 (Satellite LLC, Russia), Ecotab-Active (Novodez JSC, Russia), Septodez-Forte (Novodez, JSC Russia). As test objects, tiles, ceramics, linoleum, painted wood, lining oilcloth, linen with and without sick discharge, dishes with and without food residues, medical products made of glass, plastic, rubber, silicone and metal were used. The criterion for the activity of the disin¬fectant was the lack of growth of microorganisms on solid and liquid nutrient media. To achieve statistical reliability, all tests were performed three times. Results and discussion. The conducted studies allow us to recommend B. pseudomallei 97 and B. thailandensis 264 as test strains for evaluating the bactericidal activity of new disinfectants in the laboratory against glanders and melioidosis infections. All studied disinfectants have a pronounced activity against glanders and melioidosis pathogens and can be used in concentrations from 0.1 to 2.5 %, for 60 minutes exposure.