Electronic signals are electrogenetically relayed to control cell growth and co-culture composition

There is much to be gained by enabling electronic interrogation and control of biological function. While the benefits of bioelectronics that rely on potential-driven ionic flows are well known (electrocardiograms, defibrillators, neural prostheses, etc) there are relatively few advances targeting n...

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Main Authors:	Kristina Stephens, Fauziah Rahma Zakaria, Eric VanArsdale, Gregory F. Payne, William E. Bentley
Format:	Article
Language:	English
Published:	Elsevier 2021-12-01
Series:	Metabolic Engineering Communications
Subjects:	Electrogenetics Quorum sensing Cell-cell signaling Microbiome Internet-of-things
Online Access:	http://www.sciencedirect.com/science/article/pii/S221403012100016X

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spelling	doaj-afe05a516a8f4da9a426da6ec0601b342021-06-19T04:54:19ZengElsevierMetabolic Engineering Communications2214-03012021-12-0113e00176Electronic signals are electrogenetically relayed to control cell growth and co-culture compositionKristina Stephens0Fauziah Rahma Zakaria1Eric VanArsdale2Gregory F. Payne3William E. Bentley4Fischell Department of Bioengineering, University of Maryland, College Park, USA; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USAFischell Department of Bioengineering, University of Maryland, College Park, USA; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USAFischell Department of Bioengineering, University of Maryland, College Park, USA; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USAInstitute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USAFischell Department of Bioengineering, University of Maryland, College Park, USA; Institute for Bioscience and Biotechnology Research, University of Maryland, College Park, USA; Robert E. Fischell Institute for Biomedical Devices, University of Maryland, College Park, USA; Corresponding author. Fischell Department of Bioengineering, 5102 Clark Hall, University of Maryland, College Park, MD, 20742, USA.There is much to be gained by enabling electronic interrogation and control of biological function. While the benefits of bioelectronics that rely on potential-driven ionic flows are well known (electrocardiograms, defibrillators, neural prostheses, etc) there are relatively few advances targeting nonionic molecular networks, including genetic circuits. Redox activities combine connectivity to electronics with the potential for specific genetic control in cells. Here, electrode-generated hydrogen peroxide is used to actuate an electrogenetic “relay” cell population, which interprets the redox cue and synthesizes a bacterial signaling molecule (quorum sensing autoinducer AI-1) that, in turn, signals increased growth rate in a second population. The dramatically increased growth rate of the second population is enabled by expression of a phosphotransferase system protein, HPr, which is important for glucose transport. The potential to electronically modulate cell growth via direct genetic control will enable new opportunities in the treatment of disease and manufacture of biological therapeutics and other molecules.http://www.sciencedirect.com/science/article/pii/S221403012100016XElectrogeneticsQuorum sensingCell-cell signalingMicrobiomeInternet-of-things
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Kristina Stephens Fauziah Rahma Zakaria Eric VanArsdale Gregory F. Payne William E. Bentley
spellingShingle	Kristina Stephens Fauziah Rahma Zakaria Eric VanArsdale Gregory F. Payne William E. Bentley Electronic signals are electrogenetically relayed to control cell growth and co-culture composition Metabolic Engineering Communications Electrogenetics Quorum sensing Cell-cell signaling Microbiome Internet-of-things
author_facet	Kristina Stephens Fauziah Rahma Zakaria Eric VanArsdale Gregory F. Payne William E. Bentley
author_sort	Kristina Stephens
title	Electronic signals are electrogenetically relayed to control cell growth and co-culture composition
title_short	Electronic signals are electrogenetically relayed to control cell growth and co-culture composition
title_full	Electronic signals are electrogenetically relayed to control cell growth and co-culture composition
title_fullStr	Electronic signals are electrogenetically relayed to control cell growth and co-culture composition
title_full_unstemmed	Electronic signals are electrogenetically relayed to control cell growth and co-culture composition
title_sort	electronic signals are electrogenetically relayed to control cell growth and co-culture composition
publisher	Elsevier
series	Metabolic Engineering Communications
issn	2214-0301
publishDate	2021-12-01
description	There is much to be gained by enabling electronic interrogation and control of biological function. While the benefits of bioelectronics that rely on potential-driven ionic flows are well known (electrocardiograms, defibrillators, neural prostheses, etc) there are relatively few advances targeting nonionic molecular networks, including genetic circuits. Redox activities combine connectivity to electronics with the potential for specific genetic control in cells. Here, electrode-generated hydrogen peroxide is used to actuate an electrogenetic “relay” cell population, which interprets the redox cue and synthesizes a bacterial signaling molecule (quorum sensing autoinducer AI-1) that, in turn, signals increased growth rate in a second population. The dramatically increased growth rate of the second population is enabled by expression of a phosphotransferase system protein, HPr, which is important for glucose transport. The potential to electronically modulate cell growth via direct genetic control will enable new opportunities in the treatment of disease and manufacture of biological therapeutics and other molecules.
topic	Electrogenetics Quorum sensing Cell-cell signaling Microbiome Internet-of-things
url	http://www.sciencedirect.com/science/article/pii/S221403012100016X
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Electronic signals are electrogenetically relayed to control cell growth and co-culture composition

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