Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease

Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease

Studying Parkinson's disease (PD), one of the most common neurodegenerative disorders worldwide, requires different model systems, including patient-specific induced pluripotent stem cell lines. With the help of non-integrating episomal vectors the iPSC lines ICGi015-A and ICGi015-B were genera...

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Main Authors: D.V. Sharipova, V.R. Kovalenko, E.M. Bairamova, V.A. Vartanova, E.V. Grigor'eva, Y.V. Vyatkin, E.A. Khabarova, D.A. Rzaev, S.M. Zakian, S.P. Medvedev
Format: Article
Language:English
Published: Elsevier 2019-12-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S187350611930282X
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spelling doaj-af98c3477237437ca36c2b9079e960812020-11-25T00:53:57ZengElsevierStem Cell Research1873-50612019-12-0141Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's diseaseD.V. Sharipova0V.R. Kovalenko1E.M. Bairamova2V.A. Vartanova3E.V. Grigor'eva4Y.V. Vyatkin5E.A. Khabarova6D.A. Rzaev7S.M. Zakian8S.P. Medvedev9Federal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, RussiaFederal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaFederal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, RussiaFederal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, RussiaFederal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, RussiaNovosibirsk State University, Novosibirsk, Russia; AcademGene LLC, Novosibirsk, Russia; St. Laurent Institute, Woburn, USAFederal Neurosurgical Center, Novosibirsk, RussiaFederal Neurosurgical Center, Novosibirsk, RussiaFederal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, RussiaFederal Research Center Institute of Cytology and Genetics, the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia; E.N. Meshalkin National Medical Research Center, Ministry of Health of the Russian Federation, Novosibirsk, Russia; Novosibirsk State University, Novosibirsk, Russia; Corresponding author at: Laboratory of developmental epigenetics, Lavrentyev ave., 10, 630090 Novosibirsk, Russia.Studying Parkinson's disease (PD), one of the most common neurodegenerative disorders worldwide, requires different model systems, including patient-specific induced pluripotent stem cell lines. With the help of non-integrating episomal vectors the iPSC lines ICGi015-A and ICGi015-B were generated from blood mononuclear cells of PD patient, carrying three SNPs, associated with PD development. The obtained iPSC lines express pluripotency markers and demonstrate the ability to in vitro differentiate into the three germ layers. These cell lines may be useful for studying molecular mechanisms of PD and for drug screening.http://www.sciencedirect.com/science/article/pii/S187350611930282X
collection DOAJ
language English
format Article
sources DOAJ
author D.V. Sharipova
V.R. Kovalenko
E.M. Bairamova
V.A. Vartanova
E.V. Grigor'eva
Y.V. Vyatkin
E.A. Khabarova
D.A. Rzaev
S.M. Zakian
S.P. Medvedev
spellingShingle D.V. Sharipova
V.R. Kovalenko
E.M. Bairamova
V.A. Vartanova
E.V. Grigor'eva
Y.V. Vyatkin
E.A. Khabarova
D.A. Rzaev
S.M. Zakian
S.P. Medvedev
Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease
Stem Cell Research
author_facet D.V. Sharipova
V.R. Kovalenko
E.M. Bairamova
V.A. Vartanova
E.V. Grigor'eva
Y.V. Vyatkin
E.A. Khabarova
D.A. Rzaev
S.M. Zakian
S.P. Medvedev
author_sort D.V. Sharipova
title Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease
title_short Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease
title_full Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease
title_fullStr Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease
title_full_unstemmed Generation of two iPSC lines, (ICGi015-A and ICGi015-B), by reprogramming peripheral blood mononuclear cells from a patient with Parkinson's disease
title_sort generation of two ipsc lines, (icgi015-a and icgi015-b), by reprogramming peripheral blood mononuclear cells from a patient with parkinson's disease
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2019-12-01
description Studying Parkinson's disease (PD), one of the most common neurodegenerative disorders worldwide, requires different model systems, including patient-specific induced pluripotent stem cell lines. With the help of non-integrating episomal vectors the iPSC lines ICGi015-A and ICGi015-B were generated from blood mononuclear cells of PD patient, carrying three SNPs, associated with PD development. The obtained iPSC lines express pluripotency markers and demonstrate the ability to in vitro differentiate into the three germ layers. These cell lines may be useful for studying molecular mechanisms of PD and for drug screening.
url http://www.sciencedirect.com/science/article/pii/S187350611930282X
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