Diagnostic performance of direct latex agglutination, post-enrichment latex agglutination and culture methods in screening of group B streptococci in late pregnancy: a comparative study

• Main Authors: El Shahaway AA, El Maghraby HM, Mohammed HA, Abd Elhady RR, Abdelrhman AA
• Format: Article
• Language: English
• Published: Dove Medical Press 2019-08-01
• Series: Infection and Drug Resistance
• Subjects: Streptococcus agalactiae; vaginal swab; post enrichment; agglutination; neonatal infection.
• Online Access: https://www.dovepress.com/diagnostic-performance-of-direct-latex-agglutination-post-enrichment-l-peer-reviewed-article-IDR

Alia A El Shahaway,1 Hanaa M El Maghraby,1 Heba A Mohammed,1 Rasha R Abd Elhady,2 Amr Ahmed Abdelrhman21Department of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, Zagazig, Egypt; 2Department of Obstetrics and Gynecology, Faculty of Medicine, Zagazig University, Zagazig, EgyptCorrespondence: Alia A El ShahawayDepartment of Medical Microbiology and Immunology, Faculty of Medicine, Zagazig University, 12 Abdaziz Ali Street, Zagazig, Sharkia, EgyptTel [phone]Email [e-mail]: Group B streptococcus (GBS) is one of the main causes of neonatal sepsis.Purpose: Evaluation of the diagnostic performance of direct latex agglutination test (DLA), post-enrichment latex agglutination (LA) test, and direct culture on chromogenic media in rapid identification of GBS carrier in pregnant women in comparison with the conventional post-enrichment CDC-recommended culture method and further to estimate GBS carriage prevalence and its antimicrobial susceptibility.Methods: Two hundred pregnant women at gestational age (35–37 weeks) were enrolled. Three low vaginal swabs were obtained from each participant. One swab was directly inoculated into Strep B Select (SBS) agar. The second swab was inoculated in enrichment Lim broth for immunological antigen detection by post-enrichment latex agglutination (5 h and 24 h) and subculture for bacteriological detection. The third swab was used for immunological detection of GBS antigen by direct latex agglutination. The isolated GBS was subjected to antimicrobial susceptibility testing.Results: Among 200 pregnant women, 47 (23.5%) were GBS carriers. Considering post-enrichment subculture on SBS medium as a gold standard, the sensitivities for post-enrichment 5 h and 24 h LA were 66% and 95.7%, respectively. However, direct cultivation of the vaginal swabs on SBS medium and DLA recorded 83% and 4.3%, respectively, for sensitivity. All GBS isolates (100%) were sensitive to penicillin G, ampicillin, ceftriaxone, and vancomycin. In contrast, 21.3% and 12.8% of isolated GBS were resistant to erythromycin and clindamycin, respectively.Conclusion: Group B streptococcal antigen detection by latex agglutination after 5 h enrichment is a reliable, easy, and relatively rapid method for screening of GBS carriage in pregnant woman not in labor. Latex agglutination after 18–24 h enrichment can be used alternative to standard subculture method for screening GBS carriage.Keywords: Streptococcus agalactiae, vaginal swab, post-enrichment, agglutination, neonatal infection