Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line
Insecticide imidacloprid and herbicide glyphosate have a broad spectrum of applicable use in the agricultural sector of Egypt. Their ability to induce in vitro cytotoxic and oxidative stress on normal human cells (prostate epithelial WPM-Y.1 cell line) was evaluated with the methyl tetrazolium test...
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Online Access: | http://dx.doi.org/10.1155/2020/4364650 |
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doaj-af86e810d7ca424a880b3925fcb50aed2020-12-21T11:41:31ZengHindawi LimitedJournal of Toxicology1687-81911687-82052020-01-01202010.1155/2020/43646504364650Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell LineKhaled Y. Abdel-Halim0Safaa R. Osman1Mammalian & Aquatic Toxicology Department, Central Agricultural Pesticides Laboratory, Agricultural Research Center (ARC), 12618-Dokki, Giza, EgyptMammalian & Aquatic Toxicology Department, Central Agricultural Pesticides Laboratory, Agricultural Research Center (ARC), 12618-Dokki, Giza, EgyptInsecticide imidacloprid and herbicide glyphosate have a broad spectrum of applicable use in the agricultural sector of Egypt. Their ability to induce in vitro cytotoxic and oxidative stress on normal human cells (prostate epithelial WPM-Y.1 cell line) was evaluated with the methyl tetrazolium test (MTT) and histopathological investigation. Cell viability was evaluated with an MTT test for 24 h. The median inhibition concentration (IC50) values were 0.023 and 0.025 mM for imidacloprid and glyphosate, respectively. Sublethal concentrations: 1/10 and 1/50 of IC50 and IC50 levels significantly induced an increase in the lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) level compared with the untreated cells. Rapid decrease in the glutathione (GSH) content and glutathione-S-transferase (GST) activity was induced. Significant increases were recorded in activities of catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), respectively, compared with the control group. Transmission electron microscopic (TEM) investigation showed significant defects in the cells following pesticide treatments for 24 h. Therefore, it is concluded that imidacloprid and glyphosate are very toxic in vitro assays and able to induce apoptotic effects as well as oxidative stress. So, these findings provide a scenario of multibiomarkers to achieve the imposed risks of pesticides at low doses.http://dx.doi.org/10.1155/2020/4364650 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Khaled Y. Abdel-Halim Safaa R. Osman |
spellingShingle |
Khaled Y. Abdel-Halim Safaa R. Osman Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line Journal of Toxicology |
author_facet |
Khaled Y. Abdel-Halim Safaa R. Osman |
author_sort |
Khaled Y. Abdel-Halim |
title |
Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line |
title_short |
Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line |
title_full |
Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line |
title_fullStr |
Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line |
title_full_unstemmed |
Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line |
title_sort |
cytotoxicity and oxidative stress responses of imidacloprid and glyphosate in human prostate epithelial wpm-y.1 cell line |
publisher |
Hindawi Limited |
series |
Journal of Toxicology |
issn |
1687-8191 1687-8205 |
publishDate |
2020-01-01 |
description |
Insecticide imidacloprid and herbicide glyphosate have a broad spectrum of applicable use in the agricultural sector of Egypt. Their ability to induce in vitro cytotoxic and oxidative stress on normal human cells (prostate epithelial WPM-Y.1 cell line) was evaluated with the methyl tetrazolium test (MTT) and histopathological investigation. Cell viability was evaluated with an MTT test for 24 h. The median inhibition concentration (IC50) values were 0.023 and 0.025 mM for imidacloprid and glyphosate, respectively. Sublethal concentrations: 1/10 and 1/50 of IC50 and IC50 levels significantly induced an increase in the lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) level compared with the untreated cells. Rapid decrease in the glutathione (GSH) content and glutathione-S-transferase (GST) activity was induced. Significant increases were recorded in activities of catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), respectively, compared with the control group. Transmission electron microscopic (TEM) investigation showed significant defects in the cells following pesticide treatments for 24 h. Therefore, it is concluded that imidacloprid and glyphosate are very toxic in vitro assays and able to induce apoptotic effects as well as oxidative stress. So, these findings provide a scenario of multibiomarkers to achieve the imposed risks of pesticides at low doses. |
url |
http://dx.doi.org/10.1155/2020/4364650 |
work_keys_str_mv |
AT khaledyabdelhalim cytotoxicityandoxidativestressresponsesofimidaclopridandglyphosateinhumanprostateepithelialwpmy1cellline AT safaarosman cytotoxicityandoxidativestressresponsesofimidaclopridandglyphosateinhumanprostateepithelialwpmy1cellline |
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