Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing

Cytidine base editors are powerful tools for making subtle genome alterations. Here the authors analyse edited human iPSCs with whole genome sequencing and reveal the spectrum of off-target effects.

Bibliographic Details
Main Authors:	Erica McGrath, Hyunsu Shin, Linyi Zhang, Je-Nie Phue, Wells W. Wu, Rong-Fong Shen, Yoon-Young Jang, Javier Revollo, Zhaohui Ye
Format:	Article
Language:	English
Published:	Nature Publishing Group 2019-11-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-019-13342-8

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spelling	doaj-af712862da014df4afe9e07f5f556c662021-05-11T11:38:04ZengNature Publishing GroupNature Communications2041-17232019-11-011011910.1038/s41467-019-13342-8Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencingErica McGrath0Hyunsu Shin1Linyi Zhang2Je-Nie Phue3Wells W. Wu4Rong-Fong Shen5Yoon-Young Jang6Javier Revollo7Zhaohui Ye8Division of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug AdministrationDivision of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug AdministrationDivision of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug AdministrationFacility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food and Drug AdministrationFacility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food and Drug AdministrationFacility for Biotechnology Resources, Center for Biologics Evaluation and Research, Food and Drug AdministrationDepartment of Oncology, Johns Hopkins School of MedicineDivision of Genetic and Molecular Toxicology, National Center for Toxicology Research, Food and Drug AdministrationDivision of Cellular and Gene Therapies, Office of Tissues and Advanced Therapies, Center for Biologics Evaluation and Research, Food and Drug AdministrationCytidine base editors are powerful tools for making subtle genome alterations. Here the authors analyse edited human iPSCs with whole genome sequencing and reveal the spectrum of off-target effects.https://doi.org/10.1038/s41467-019-13342-8
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Erica McGrath Hyunsu Shin Linyi Zhang Je-Nie Phue Wells W. Wu Rong-Fong Shen Yoon-Young Jang Javier Revollo Zhaohui Ye
spellingShingle	Erica McGrath Hyunsu Shin Linyi Zhang Je-Nie Phue Wells W. Wu Rong-Fong Shen Yoon-Young Jang Javier Revollo Zhaohui Ye Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing Nature Communications
author_facet	Erica McGrath Hyunsu Shin Linyi Zhang Je-Nie Phue Wells W. Wu Rong-Fong Shen Yoon-Young Jang Javier Revollo Zhaohui Ye
author_sort	Erica McGrath
title	Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing
title_short	Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing
title_full	Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing
title_fullStr	Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing
title_full_unstemmed	Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing
title_sort	targeting specificity of apobec-based cytosine base editor in human ipscs determined by whole genome sequencing
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2019-11-01
description	Cytidine base editors are powerful tools for making subtle genome alterations. Here the authors analyse edited human iPSCs with whole genome sequencing and reveal the spectrum of off-target effects.
url	https://doi.org/10.1038/s41467-019-13342-8
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Targeting specificity of APOBEC-based cytosine base editor in human iPSCs determined by whole genome sequencing

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