INFLUENCE OF ENDOTHELIAL CELLS PREINCUBATED WITH CYTOKINES ON THP-1 MONOCYTE-LIKE CELLS PHENOTYPE AND FUNCTIONS

• Main Authors: E. A. Starikova, I. S. Freidlin, D. I. Sokolov, S. A. Selkov, K. A. Sisoev, Areg A. Totolian
• Format: Article
• Language: Russian
• Published: SPb RAACI 2014-07-01
• Series: Medicinskaâ Immunologiâ
• Subjects: endothelial cells; cytokines; monocytes; adhesion; migration; phenotype
• Online Access: http://mimmun.ru/index.php/mimmun/article/view/593

<p><strong>Abstract. </strong>We demonstrated that TNFα, IFNγ and IL-4 induce different patterns of endothelial cells activation. The greatest alterations were observed in TNFα threated endothelial cells. Under the influence of this cytokine there was a statistically significant elevation of IL-8 and MCP-1 production by endothelial cells, of THP-1 adhesion and of migration toward endothelial cells monolayer. At the same time we had not observed any immunophenotype alteration of THP-1 cocultured with endothelial cells treated with TNFα.</p><p>IFNγ и IL-4 induced statistically significant inhibition of THP-1 adhesion. IL-4 treated endothelial cells have elevated levels of IL-8 and MCP-1 production and stimulated THP-1 migrations. IFNγ showed inhibition of IL-8 and MCP-1 spontaneous production and THP-1migration.</p><p>Incubation of THP-1 with untreated and IFNγ treated endothelial cells led to significant elevation of HLA-DR expression. Expression of CD68 was significantly higher in case of THP-1 cocultured with IL-4 treated endothelial cells. There was no any correlation between immunophenotype alteration THP-1cells and others parameters alteration.</p>