A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell Carcinoma

Laryngeal squamous cell carcinoma (LSCC) is an aggressive type of head and neck squamous cell carcinoma (HNSCC) with a relatively high rate of morbidity and mortality. An altered miR-144-3p level in LSCC with a small number of patients has been previously reported. However, the clinical implication...

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Main Authors: Bin-Yu Mo, Jin-Shu Pang, Wen-Bin Dai, Ya-si Su, Wei Jiang, Su-Ning Huang
Format: Article
Language:English
Published: Hindawi Limited 2021-01-01
Series:Computational and Mathematical Methods in Medicine
Online Access:http://dx.doi.org/10.1155/2021/6684186
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spelling doaj-af4bb89b18f3478aaedb919ef91695892021-07-26T00:34:42ZengHindawi LimitedComputational and Mathematical Methods in Medicine1748-67182021-01-01202110.1155/2021/6684186A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell CarcinomaBin-Yu Mo0Jin-Shu Pang1Wen-Bin Dai2Ya-si Su3Wei Jiang4Su-Ning Huang5Department of OtolaryngologyDepartment of RadiotherapyDepartment of PathologyDepartment of PathologyDepartment of Educational AdministrationDepartment of RadiotherapyLaryngeal squamous cell carcinoma (LSCC) is an aggressive type of head and neck squamous cell carcinoma (HNSCC) with a relatively high rate of morbidity and mortality. An altered miR-144-3p level in LSCC with a small number of patients has been previously reported. However, the clinical implication of miR-144-3p and its involved mechanism underlying this disease is not clearly elucidated. In this work, we aimed to confirm the expression of miR-144-3p with larger samples and also to identify target genes for the investigation of the underlying mechanism of miR-144-3p in LSCC. The levels of miR-144-3p were downregulated in 155 samples of LSCC tissues as compared to 26 non-LSCC samples (SMD: -0.78; 95% confidence interval (CI): -1.23, -0.32). The AUC of 0.90 in the summarized ROC curve also indicated a potential ability to differentiate LSCC from non-LSCC tissues, with a sensitivity of 0.78 and a specificity of 0.88. With respect to the molecular mechanism, we predicted the potential targets from online-based prediction, peer-reviewed publications, and RNA-seq and microarray data. In particular, the genes influenced by transfection with miR-144-3p in the LSCC FaDu cell line were collected from the microarray GSE56243. Lastly, 12 novel targets for miR-144-3p in LSCC were obtained by different algorithms. In conclusion, our study confirmed the loss or downregulation of miR-144-3p in LSCC, which might contribute to the LSCC tumorigenesis and progression via regulation of the 12 novel targets, such as IL24, ITGA6, and CEP55. In the future, further investigations are required to validate the present results.http://dx.doi.org/10.1155/2021/6684186
collection DOAJ
language English
format Article
sources DOAJ
author Bin-Yu Mo
Jin-Shu Pang
Wen-Bin Dai
Ya-si Su
Wei Jiang
Su-Ning Huang
spellingShingle Bin-Yu Mo
Jin-Shu Pang
Wen-Bin Dai
Ya-si Su
Wei Jiang
Su-Ning Huang
A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell Carcinoma
Computational and Mathematical Methods in Medicine
author_facet Bin-Yu Mo
Jin-Shu Pang
Wen-Bin Dai
Ya-si Su
Wei Jiang
Su-Ning Huang
author_sort Bin-Yu Mo
title A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell Carcinoma
title_short A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell Carcinoma
title_full A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell Carcinoma
title_fullStr A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell Carcinoma
title_full_unstemmed A Comprehensive Evaluation of miR-144-3p Expression and Its Targets in Laryngeal Squamous Cell Carcinoma
title_sort comprehensive evaluation of mir-144-3p expression and its targets in laryngeal squamous cell carcinoma
publisher Hindawi Limited
series Computational and Mathematical Methods in Medicine
issn 1748-6718
publishDate 2021-01-01
description Laryngeal squamous cell carcinoma (LSCC) is an aggressive type of head and neck squamous cell carcinoma (HNSCC) with a relatively high rate of morbidity and mortality. An altered miR-144-3p level in LSCC with a small number of patients has been previously reported. However, the clinical implication of miR-144-3p and its involved mechanism underlying this disease is not clearly elucidated. In this work, we aimed to confirm the expression of miR-144-3p with larger samples and also to identify target genes for the investigation of the underlying mechanism of miR-144-3p in LSCC. The levels of miR-144-3p were downregulated in 155 samples of LSCC tissues as compared to 26 non-LSCC samples (SMD: -0.78; 95% confidence interval (CI): -1.23, -0.32). The AUC of 0.90 in the summarized ROC curve also indicated a potential ability to differentiate LSCC from non-LSCC tissues, with a sensitivity of 0.78 and a specificity of 0.88. With respect to the molecular mechanism, we predicted the potential targets from online-based prediction, peer-reviewed publications, and RNA-seq and microarray data. In particular, the genes influenced by transfection with miR-144-3p in the LSCC FaDu cell line were collected from the microarray GSE56243. Lastly, 12 novel targets for miR-144-3p in LSCC were obtained by different algorithms. In conclusion, our study confirmed the loss or downregulation of miR-144-3p in LSCC, which might contribute to the LSCC tumorigenesis and progression via regulation of the 12 novel targets, such as IL24, ITGA6, and CEP55. In the future, further investigations are required to validate the present results.
url http://dx.doi.org/10.1155/2021/6684186
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