Effect of 6 Weeks of Resistance Training and Boldenone Supplementation on 5-alpha Reductase and Aromatase Gene Expression in Testes Tissue of Male Wistar Rats

• Main Authors: M. Sadeghi, A. Abbassi Daloii, S.J. Ziaolhagh
• Format: Article
• Language: English
• Published: Gonabad University of Medical Sciences (GMU) 2017-09-01
• Series: Ufuq-i Dānish
• Subjects: Resistance Training; Boldenone
• Online Access: http://hms.gmu.ac.ir/article-1-2549-en.pdf
• ISSN: 1735-1855; 2252-0805

Aims: Using anabolic androgenic steroids by athletes has significant side effects on sex hormones and the reproductive system. The aim of this study was to investigate the effect of 6 weeks of resistance exercise and Boldenone supplements on the expression of 5-alpha reductase and aromatase genes of the testis tissue in Wistar rats. Material & Methods: In this experimental study, thirty 12-week old male Wistar rats with the average weight of 195.00±7.94 grams were divided randomly into 5 groups; control, sham, Boldenone supplements (2mg per each kilogram of body weight), resistance exercise and Boldenone exercise. Resistance exercise program was 5 sessions of climbing the ladder each week (3 sets of 5 repeats) for 6 weeks that was started by 50% of one maximum repetition and reached 100% at the end. The level of 5-alpha reductase and aromatase genes expression were measured after the anesthesia and the removal of the testes tissue in the samples. Data was analyzed by paired T, ANOVA and Tukey post hoc tests by using SPSS 22 software. Findings: The average weight of all groups’ mice were significantly increased in week 6 comparing to the first week (p=0.0001). There was significant increasing in 5-alpha-reductase expression in Boldenone and Boldenone exercise than the control group and also in the Boldenone exercise than resistance exercise group after the intervention. There was significant increasing in aromatase gene expression in resistance exercise and Boldenone exercise groups than the control group (p<0.05). Conclusion: Boldenone supplement along with 6 weeks of resistance exercise increases the levels of 5-alpha reductase and aromatase genes expression in testis tissue of Wistar rats.