Quantifying Fluorescently Labeled Ceramide Levels in Human Sarcoma Cell Lines in Response to a Sphingomyelin Synthase Inhibitor

Quantifying Fluorescently Labeled Ceramide Levels in Human Sarcoma Cell Lines in Response to a Sphingomyelin Synthase Inhibitor

Sphingolipid metabolism is an important process in sustaining the growth needs of rapidly dividing cancer cells. Enzymes that synthesize sphingolipids have become attractive targets in cancer pharmacology. Ceramide is a precursor for synthesizing sphingolipids such as sphingomyelin, sphingosine-1-ph...

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Bibliographic Details
Main Authors: Srinath Pashikanti, Farjana Afrin, Trevor C. Meldrum, John L. Stegelmeier, Adriene Pavek, Yashar A. Habashi, Kaniz Fatema, Jared J. Barrott
Format: Article
Language:English
Published: MDPI AG 2019-08-01
Series:Methods and Protocols
Subjects:
ceramide
synovial sarcoma
osteosarcoma
sphingomyelin synthase inhibitor
jaspine B
cellular assay
Online Access:https://www.mdpi.com/2409-9279/2/3/76

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