Differential reactivity of human low density lipoproteins with monoclonal antibodies.

Differential reactivity of human low density lipoproteins with monoclonal antibodies.

The immunoreactivities of LDL (low density lipoprotein) samples obtained from a variety of subjects were analyzed by comparing their capacities to compete with 125I-labeled LDL for binding to various monoclonal anti-LDL antibodies in competitive binding assays. A marked variation in epitope expressi...

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Main Authors: M J Tikkanen, T G Cole, G Schonfeld
Format: Article
Language:English
Published: Elsevier 1983-11-01
Series:Journal of Lipid Research
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520378731
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spelling doaj-aef9d6f9964f4ba58709f5cdda1e00e52021-04-25T04:17:01ZengElsevierJournal of Lipid Research0022-22751983-11-01241114941499Differential reactivity of human low density lipoproteins with monoclonal antibodies.M J TikkanenT G ColeG SchonfeldThe immunoreactivities of LDL (low density lipoprotein) samples obtained from a variety of subjects were analyzed by comparing their capacities to compete with 125I-labeled LDL for binding to various monoclonal anti-LDL antibodies in competitive binding assays. A marked variation in epitope expression was observed. In comparison to an LDL standard, different preparations exhibited immunoreactivities (expressed as apparent apoB content) ranging from 30 to 400% of the LDL standard. Some epitopes were much more uniformly expressed than others. The number of epitopes expressed in different LDL preparations appeared to be related to the percentage composition of various lipid constituents in LDL. The results support the hypothesis that the epitope expression of apoB is modulated by the composition of the lipids associated with it.http://www.sciencedirect.com/science/article/pii/S0022227520378731
collection DOAJ
language English
format Article
sources DOAJ
author M J Tikkanen
T G Cole
G Schonfeld
spellingShingle M J Tikkanen
T G Cole
G Schonfeld
Differential reactivity of human low density lipoproteins with monoclonal antibodies.
Journal of Lipid Research
author_facet M J Tikkanen
T G Cole
G Schonfeld
author_sort M J Tikkanen
title Differential reactivity of human low density lipoproteins with monoclonal antibodies.
title_short Differential reactivity of human low density lipoproteins with monoclonal antibodies.
title_full Differential reactivity of human low density lipoproteins with monoclonal antibodies.
title_fullStr Differential reactivity of human low density lipoproteins with monoclonal antibodies.
title_full_unstemmed Differential reactivity of human low density lipoproteins with monoclonal antibodies.
title_sort differential reactivity of human low density lipoproteins with monoclonal antibodies.
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1983-11-01
description The immunoreactivities of LDL (low density lipoprotein) samples obtained from a variety of subjects were analyzed by comparing their capacities to compete with 125I-labeled LDL for binding to various monoclonal anti-LDL antibodies in competitive binding assays. A marked variation in epitope expression was observed. In comparison to an LDL standard, different preparations exhibited immunoreactivities (expressed as apparent apoB content) ranging from 30 to 400% of the LDL standard. Some epitopes were much more uniformly expressed than others. The number of epitopes expressed in different LDL preparations appeared to be related to the percentage composition of various lipid constituents in LDL. The results support the hypothesis that the epitope expression of apoB is modulated by the composition of the lipids associated with it.
url http://www.sciencedirect.com/science/article/pii/S0022227520378731
work_keys_str_mv AT mjtikkanen differentialreactivityofhumanlowdensitylipoproteinswithmonoclonalantibodies
AT tgcole differentialreactivityofhumanlowdensitylipoproteinswithmonoclonalantibodies
AT gschonfeld differentialreactivityofhumanlowdensitylipoproteinswithmonoclonalantibodies
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