Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms

CXCR2 is central to neutrophil chemotaxis and hence to some inflammatory diseases. Here the authors demonstrate the value of an epitope-guided antibody panning method to develop a tight binding anti-hCXCR2 antibody, along with crystal structures of this antibody and antigen, that can block neutrophi...

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Main Authors:	Xiaojie Shi, Yue Wan, Nan Wang, Jiangchao Xiang, Tao Wang, Xiaofeng Yang, Ju Wang, Xuxue Dong, Liang Dong, Lei Yan, Yu Li, Lili Liu, Shinchen Hou, Zhenwei Zhong, Ian A. Wilson, Bei Yang, Guang Yang, Richard A. Lerner
Format:	Article
Language:	English
Published:	Nature Publishing Group 2021-05-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-021-22810-z

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spelling	doaj-aeb13ed177694e308a13ce3af41fa8c92021-05-09T11:12:08ZengNature Publishing GroupNature Communications2041-17232021-05-0112111410.1038/s41467-021-22810-zSelection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptomsXiaojie Shi0Yue Wan1Nan Wang2Jiangchao Xiang3Tao Wang4Xiaofeng Yang5Ju Wang6Xuxue Dong7Liang Dong8Lei Yan9Yu Li10Lili Liu11Shinchen Hou12Zhenwei Zhong13Ian A. Wilson14Bei Yang15Guang Yang16Richard A. Lerner17Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityDepartment of Integrative Structural and Computational Biology, The Scripps Research Institute, La JollaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech UniversityCXCR2 is central to neutrophil chemotaxis and hence to some inflammatory diseases. Here the authors demonstrate the value of an epitope-guided antibody panning method to develop a tight binding anti-hCXCR2 antibody, along with crystal structures of this antibody and antigen, that can block neutrophil chemotaxis and protect mice in an EAE model.https://doi.org/10.1038/s41467-021-22810-z
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Xiaojie Shi Yue Wan Nan Wang Jiangchao Xiang Tao Wang Xiaofeng Yang Ju Wang Xuxue Dong Liang Dong Lei Yan Yu Li Lili Liu Shinchen Hou Zhenwei Zhong Ian A. Wilson Bei Yang Guang Yang Richard A. Lerner
spellingShingle	Xiaojie Shi Yue Wan Nan Wang Jiangchao Xiang Tao Wang Xiaofeng Yang Ju Wang Xuxue Dong Liang Dong Lei Yan Yu Li Lili Liu Shinchen Hou Zhenwei Zhong Ian A. Wilson Bei Yang Guang Yang Richard A. Lerner Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms Nature Communications
author_facet	Xiaojie Shi Yue Wan Nan Wang Jiangchao Xiang Tao Wang Xiaofeng Yang Ju Wang Xuxue Dong Liang Dong Lei Yan Yu Li Lili Liu Shinchen Hou Zhenwei Zhong Ian A. Wilson Bei Yang Guang Yang Richard A. Lerner
author_sort	Xiaojie Shi
title	Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms
title_short	Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms
title_full	Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms
title_fullStr	Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms
title_full_unstemmed	Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms
title_sort	selection of a picomolar antibody that targets cxcr2-mediated neutrophil activation and alleviates eae symptoms
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2021-05-01
description	CXCR2 is central to neutrophil chemotaxis and hence to some inflammatory diseases. Here the authors demonstrate the value of an epitope-guided antibody panning method to develop a tight binding anti-hCXCR2 antibody, along with crystal structures of this antibody and antigen, that can block neutrophil chemotaxis and protect mice in an EAE model.
url	https://doi.org/10.1038/s41467-021-22810-z
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Selection of a picomolar antibody that targets CXCR2-mediated neutrophil activation and alleviates EAE symptoms

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