High prevalence of the EBER variant EB-8m in endemic nasopharyngeal carcinomas.

Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) are the most highly expressed transcripts in all EBV-associated tumors and are involved in both lymphoid and epithelioid carcinogenesis. Our previous study on Chinese isolates from non-endemic area of nasopharyngeal carcinoma (NPC) identified new E...

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Main Authors: Zhi-chao Shen, Bing Luo, Jian-ning Chen, Yan Chao, Chun-kui Shao, Qian-qian Liu, Yun Wang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4373760?pdf=render
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spelling doaj-ae7b953d2c3845e4ac74eef4bdb5863b2020-11-24T21:59:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e012142010.1371/journal.pone.0121420High prevalence of the EBER variant EB-8m in endemic nasopharyngeal carcinomas.Zhi-chao ShenBing LuoJian-ning ChenYan ChaoChun-kui ShaoQian-qian LiuYun WangEpstein-Barr virus (EBV)-encoded small RNAs (EBERs) are the most highly expressed transcripts in all EBV-associated tumors and are involved in both lymphoid and epithelioid carcinogenesis. Our previous study on Chinese isolates from non-endemic area of nasopharyngeal carcinoma (NPC) identified new EBER variants (EB-8m and EB-10m) which were less common but relatively more frequent in NPC cases than healthy donors. In the present study, we determined the EBER variants in NPC cases and healthy donors from endemic and non-endemic areas of NPC within China and compared the EBER variants, in relation to the genotypes at BamHI F region (prototype F and f variant), between population groups and between two areas. According to the phylogenetic tree, four EBER variants (EB-6m, EB-8m, EB-10m and B95-8) were identified. EB-6m was dominant in all population groups except for endemic NPC group, in which EB-8m was dominant. EB-8m was more common in endemic NPC cases (82.0%, 41/50) than non-endemic NPC cases (33.7%, 32/95) (p<0.0001), and it was also more frequent in healthy donors from endemic area (32.4%, 24/74) than healthy donors from non-endemic area (1.1%, 1/92) (p<0.0001). More importantly, the EB-8m was more prevalent in NPC cases than healthy donors in both areas (p<0.0001). The f variant, which has been suggested to associate with endemic NPC, demonstrated preferential linkage with EB-8m in endemic isolates, however, the EB-8m variant seemed to be more specific to NPC isolates than f variant. These results reveal high prevalence of EBER EB-8m variant in endemic NPC cases, suggesting an association between NPC development and EBV isolates carrying EB-8m variant. Our finding identified a small healthy population group that shares the same viral strain which predominates in NPC cases. It could be interesting to carry extensive cohort studies following these individuals to evaluate the risk to develop NPC.http://europepmc.org/articles/PMC4373760?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Zhi-chao Shen
Bing Luo
Jian-ning Chen
Yan Chao
Chun-kui Shao
Qian-qian Liu
Yun Wang
spellingShingle Zhi-chao Shen
Bing Luo
Jian-ning Chen
Yan Chao
Chun-kui Shao
Qian-qian Liu
Yun Wang
High prevalence of the EBER variant EB-8m in endemic nasopharyngeal carcinomas.
PLoS ONE
author_facet Zhi-chao Shen
Bing Luo
Jian-ning Chen
Yan Chao
Chun-kui Shao
Qian-qian Liu
Yun Wang
author_sort Zhi-chao Shen
title High prevalence of the EBER variant EB-8m in endemic nasopharyngeal carcinomas.
title_short High prevalence of the EBER variant EB-8m in endemic nasopharyngeal carcinomas.
title_full High prevalence of the EBER variant EB-8m in endemic nasopharyngeal carcinomas.
title_fullStr High prevalence of the EBER variant EB-8m in endemic nasopharyngeal carcinomas.
title_full_unstemmed High prevalence of the EBER variant EB-8m in endemic nasopharyngeal carcinomas.
title_sort high prevalence of the eber variant eb-8m in endemic nasopharyngeal carcinomas.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2015-01-01
description Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) are the most highly expressed transcripts in all EBV-associated tumors and are involved in both lymphoid and epithelioid carcinogenesis. Our previous study on Chinese isolates from non-endemic area of nasopharyngeal carcinoma (NPC) identified new EBER variants (EB-8m and EB-10m) which were less common but relatively more frequent in NPC cases than healthy donors. In the present study, we determined the EBER variants in NPC cases and healthy donors from endemic and non-endemic areas of NPC within China and compared the EBER variants, in relation to the genotypes at BamHI F region (prototype F and f variant), between population groups and between two areas. According to the phylogenetic tree, four EBER variants (EB-6m, EB-8m, EB-10m and B95-8) were identified. EB-6m was dominant in all population groups except for endemic NPC group, in which EB-8m was dominant. EB-8m was more common in endemic NPC cases (82.0%, 41/50) than non-endemic NPC cases (33.7%, 32/95) (p<0.0001), and it was also more frequent in healthy donors from endemic area (32.4%, 24/74) than healthy donors from non-endemic area (1.1%, 1/92) (p<0.0001). More importantly, the EB-8m was more prevalent in NPC cases than healthy donors in both areas (p<0.0001). The f variant, which has been suggested to associate with endemic NPC, demonstrated preferential linkage with EB-8m in endemic isolates, however, the EB-8m variant seemed to be more specific to NPC isolates than f variant. These results reveal high prevalence of EBER EB-8m variant in endemic NPC cases, suggesting an association between NPC development and EBV isolates carrying EB-8m variant. Our finding identified a small healthy population group that shares the same viral strain which predominates in NPC cases. It could be interesting to carry extensive cohort studies following these individuals to evaluate the risk to develop NPC.
url http://europepmc.org/articles/PMC4373760?pdf=render
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