Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning
Summary: Local translation is a conserved mechanism conferring cells the ability to quickly respond to local stimuli. In the brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet the specificity and regulation of astrocyte local translation...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-08-01
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| Series: | Cell Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2211124720310615 |
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doaj-ae761e66c5cc40cfbcdcc3b57bcb4336 |
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Article |
| collection |
DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Noémie Mazaré Marc Oudart Julien Moulard Giselle Cheung Romain Tortuyaux Philippe Mailly David Mazaud Alexis-Pierre Bemelmans Anne-Cécile Boulay Corinne Blugeon Laurent Jourdren Stéphane Le Crom Nathalie Rouach Martine Cohen-Salmon |
| spellingShingle |
Noémie Mazaré Marc Oudart Julien Moulard Giselle Cheung Romain Tortuyaux Philippe Mailly David Mazaud Alexis-Pierre Bemelmans Anne-Cécile Boulay Corinne Blugeon Laurent Jourdren Stéphane Le Crom Nathalie Rouach Martine Cohen-Salmon Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning Cell Reports astrocyte perisynaptic astrocyte processes local translation neuroglial interactions |
| author_facet |
Noémie Mazaré Marc Oudart Julien Moulard Giselle Cheung Romain Tortuyaux Philippe Mailly David Mazaud Alexis-Pierre Bemelmans Anne-Cécile Boulay Corinne Blugeon Laurent Jourdren Stéphane Le Crom Nathalie Rouach Martine Cohen-Salmon |
| author_sort |
Noémie Mazaré |
| title |
Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning |
| title_short |
Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning |
| title_full |
Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning |
| title_fullStr |
Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning |
| title_full_unstemmed |
Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear Conditioning |
| title_sort |
local translation in perisynaptic astrocytic processes is specific and changes after fear conditioning |
| publisher |
Elsevier |
| series |
Cell Reports |
| issn |
2211-1247 |
| publishDate |
2020-08-01 |
| description |
Summary: Local translation is a conserved mechanism conferring cells the ability to quickly respond to local stimuli. In the brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet the specificity and regulation of astrocyte local translation remain unknown. We study hippocampal perisynaptic astrocytic processes (PAPs) and show that they contain the machinery for translation. Using a refined immunoprecipitation technique, we characterize the entire pool of ribosome-bound mRNAs in PAPs and compare it with the one expressed in the whole astrocyte. We find that a specific pool of mRNAs is highly polarized at the synaptic interface. These transcripts encode an unexpected molecular repertoire, composed of proteins involved in iron homeostasis, translation, cell cycle, and cytoskeleton. Remarkably, we observe alterations in global RNA distribution and ribosome-bound status of some PAP-enriched transcripts after fear conditioning, indicating the role of astrocytic local translation in memory and learning. |
| topic |
astrocyte perisynaptic astrocyte processes local translation neuroglial interactions |
| url |
http://www.sciencedirect.com/science/article/pii/S2211124720310615 |
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doaj-ae761e66c5cc40cfbcdcc3b57bcb43362020-11-25T03:51:34ZengElsevierCell Reports2211-12472020-08-01328108076Local Translation in Perisynaptic Astrocytic Processes Is Specific and Changes after Fear ConditioningNoémie Mazaré0Marc Oudart1Julien Moulard2Giselle Cheung3Romain Tortuyaux4Philippe Mailly5David Mazaud6Alexis-Pierre Bemelmans7Anne-Cécile Boulay8Corinne Blugeon9Laurent Jourdren10Stéphane Le Crom11Nathalie Rouach12Martine Cohen-Salmon13Physiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France; Doctoral School No. 158, Pierre and Marie Curie University, 75005 Paris, FrancePhysiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France; Doctoral School No. 158, Pierre and Marie Curie University, 75005 Paris, FranceDoctoral School No. 158, Pierre and Marie Curie University, 75005 Paris, France; Neuroglial Interactions in Cerebral Physiopathology Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, FranceNeuroglial Interactions in Cerebral Physiopathology Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, FrancePhysiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, FranceOrion Imaging Facility, Center for Interdisciplinary Research in Biology (CIRB), College de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, FranceNeuroglial Interactions in Cerebral Physiopathology Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, FranceCEA, DRF, Institut de Biologie François Jacob, Molecular Imaging Research Center (MIRCen), 92265 Fontenay-aux-Roses, France; CNRS, CEA, Université Paris-Sud, Université Paris-Saclay, Neurodegenerative Diseases Laboratory (UMR9199), 92265 Fontenay-aux-Roses, FrancePhysiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, FranceGenomic Facility, Institut de Biologie de l’ENS (IBENS), Département de Biologie, École Normale Supérieure, CNRS, INSERM, Université PSL, 75005 Paris, FranceGenomic Facility, Institut de Biologie de l’ENS (IBENS), Département de Biologie, École Normale Supérieure, CNRS, INSERM, Université PSL, 75005 Paris, FranceGenomic Facility, Institut de Biologie de l’ENS (IBENS), Département de Biologie, École Normale Supérieure, CNRS, INSERM, Université PSL, 75005 Paris, France; Sorbonne Université, CNRS, Institut de Biologie Paris-Seine (IBPS), Laboratory of Computational and Quantitative Biology (LCQB), 75005 Paris, FranceNeuroglial Interactions in Cerebral Physiopathology Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, FrancePhysiology and Physiopathology of the Gliovascular Unit Research Group, Center for Interdisciplinary Research in Biology (CIRB), Collège de France, CNRS Unité Mixte de Recherche 724, INSERM Unité 1050, Labex Memolife, PSL Research University, Paris, France; Corresponding authorSummary: Local translation is a conserved mechanism conferring cells the ability to quickly respond to local stimuli. In the brain, it has been recently reported in astrocytes, whose fine processes contact blood vessels and synapses. Yet the specificity and regulation of astrocyte local translation remain unknown. We study hippocampal perisynaptic astrocytic processes (PAPs) and show that they contain the machinery for translation. Using a refined immunoprecipitation technique, we characterize the entire pool of ribosome-bound mRNAs in PAPs and compare it with the one expressed in the whole astrocyte. We find that a specific pool of mRNAs is highly polarized at the synaptic interface. These transcripts encode an unexpected molecular repertoire, composed of proteins involved in iron homeostasis, translation, cell cycle, and cytoskeleton. Remarkably, we observe alterations in global RNA distribution and ribosome-bound status of some PAP-enriched transcripts after fear conditioning, indicating the role of astrocytic local translation in memory and learning.http://www.sciencedirect.com/science/article/pii/S2211124720310615astrocyteperisynaptic astrocyte processeslocal translationneuroglial interactions |