3D Digital Anatomic Angioarchitecture of the Rat Spinal Cord: A Synchrotron Radiation Micro-CT Study

• Main Authors: Ping Li, Yan Xu, Yong Cao, Tianding Wu
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2020-07-01
• Series: Frontiers in Neuroanatomy
• Subjects: spinal cord microvasculature; SRμCT; digital anatomy; virtual micro-endoscopy; 3D print
• Online Access: https://www.frontiersin.org/article/10.3389/fnana.2020.00041/full

Comprehensive analysis of 3D angioarchitecture within the intact rat spinal cord remains technically challenging due to its sophisticated anatomical properties. In this study, we aim to present a framework for ultrahigh-resolution digitalized mapping of the normal rat spinal cord angioarchitecture and to determine the physiological parameters using synchrotron radiation micro-CT (SRμCT). Male SD rats were used in this ex vivo study. After a proportional mixture of contrast agents perfusion, the intact spinal cord covered the cervical spinal from the upper of the 1st cervical vertebra to the 5th lumbar vertebra was harvested and cut into proper lengths within three distinct regions: Cervical 3–5 levels, Thoracic 10–12 levels, Lumbar 3–5 levels spinal cord and examined using SRμCT. This method enabled the replication of the complicated microvasculature network of the normal rat spinal cord at the ultrahigh-resolution level, allowing for the precise quantitative analysis of the vascular morphological difference among cervical, thoracic and lumbar spinal cord in a 3D manner. Apart from a series of delicate 3D digital anatomical maps of the rat spinal cord angioarchitecture ranging from the cervical and thoracic to the lumbar spinal cord were presented, the 3D reconstruction data of SRμCT made the 3D printing of the spinal cord targeted selected microvasculature reality, that possibly provided deep insight into the nature and role of spinal cord intricate angioarchitecture. Our data proposed a new approach to outline systematic visual and quantitative evaluations on the 3D arrangement of the entire hierarchical microvasculature of the normal rat spinal cord at ultrahigh resolution. The technique may have great potential and become useful for future research on the poorly understood nature and function of the neurovascular interaction, particularly to investigate their pathology changes in various models of neurovascular disease.