Argyrophilic nucleolar organizer region in MIB-1 positive cells in non-small cell lung cancer: clinicopathological significance and survival

• Main Authors: Dmitriy Sergeevich Kobyakov, Ashot Merudzhanovich Avdalyan, Aleksandr Fedorovich Lazarev, Elena Leonidovna Lushnikova, Lev Moiseevich Nepomnyashchikh
• Format: Article
• Language: English
• Published: China Anti-Cancer Association 2014-12-01
• Series: Cancer Biology & Medicine
• Subjects: Argyrophilic nucleolar organizer region (AgNOR); MIB-1; survival; non-small cell lung cancer (NSCLC)
• Online Access: http://www.cancerbiomed.org/index.php/cocr/article/view/824

<b>Objective</b> To evaluate the relation between argyrophilic nucleolar organizer region (AgNOR)-associated proteins and clinicopathological parameters and survival in non-small-cell lung cancer (NSCLC). <br/><b>Methods</b> A total of 207 surgical specimens diagnosed as NSCLC were included in this study. Double-staining procedures were performed using antigen Ki-67 (clone MIB-1) and silver nitrate by immunohistochemical and AgNOR-staining methods. <br/><b>Results</b> The AgNOR area in MIB-1-positive cells of NSCLC is related to clinicopathological parameters under the TNM (tumor, node, and metastasis) system. The survival of patients with small AgNOR area in MIB-1-positive cells is better than that of patients with large AgNOR area. Molecular, biological (AgNOR area in MIB-1-positive cells), and clinicopathological (greatest tumor dimension, metastases to regional lymph nodes, histology, and differentiation) parameters are independent prognostic factors of NSCLC. <br/><b>Conclusion</b> The AgNOR area in MIB-1-positive cells is related to clinicopathological parameters and survival in NSCLC.