Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil

Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil

Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic...

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Main Authors: YUDHIE ISTIANTO, RADEN SETYO ADJI KOESOEMOWIDODO, YOSHIO WATANABE, HARDANING PRANAMUDA, BAMBANG MARWOTO
Format: Article
Language:English
Published: Indonesian Society for Microbiology 2012-04-01
Series:Microbiology Indonesia
Subjects:
phenol method
rare-Actinomycetes
selective isolation method
soil sample.
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/163
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spelling doaj-ae5ef67a58ab49ffb69dfe7c98af38e02021-08-20T12:58:39ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752012-04-016110.5454/mi.6.1.7Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian SoilYUDHIE ISTIANTORADEN SETYO ADJI KOESOEMOWIDODOYOSHIO WATANABEHARDANING PRANAMUDABAMBANG MARWOTO Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively. https://jurnal.permi.or.id/index.php/mionline/article/view/163phenol methodrare-Actinomycetesselective isolation methodsoil sample.
collection DOAJ
language English
format Article
sources DOAJ
author YUDHIE ISTIANTO
RADEN SETYO ADJI KOESOEMOWIDODO
YOSHIO WATANABE
HARDANING PRANAMUDA
BAMBANG MARWOTO
spellingShingle YUDHIE ISTIANTO
RADEN SETYO ADJI KOESOEMOWIDODO
YOSHIO WATANABE
HARDANING PRANAMUDA
BAMBANG MARWOTO
Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
Microbiology Indonesia
phenol method
rare-Actinomycetes
selective isolation method
soil sample.
author_facet YUDHIE ISTIANTO
RADEN SETYO ADJI KOESOEMOWIDODO
YOSHIO WATANABE
HARDANING PRANAMUDA
BAMBANG MARWOTO
author_sort YUDHIE ISTIANTO
title Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
title_short Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
title_full Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
title_fullStr Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
title_full_unstemmed Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil
title_sort application of phenol pretreatment for the isolation of rare actinomycetes from indonesian soil
publisher Indonesian Society for Microbiology
series Microbiology Indonesia
issn 1978-3477
2087-8575
publishDate 2012-04-01
description Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.
topic phenol method
rare-Actinomycetes
selective isolation method
soil sample.
url https://jurnal.permi.or.id/index.php/mionline/article/view/163
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AT yoshiowatanabe applicationofphenolpretreatmentfortheisolationofrareactinomycetesfromindonesiansoil
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