Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil

Application of Phenol Pretreatment for the Isolation of Rare Actinomycetes from Indonesian Soil

Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic...

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Bibliographic Details
Main Authors: YUDHIE ISTIANTO, RADEN SETYO ADJI KOESOEMOWIDODO, YOSHIO WATANABE, HARDANING PRANAMUDA, BAMBANG MARWOTO
Format: Article
Language:English
Published: Indonesian Society for Microbiology 2012-04-01
Series:Microbiology Indonesia
Subjects:
phenol method
rare-Actinomycetes
selective isolation method
soil sample.
Online Access:https://jurnal.permi.or.id/index.php/mionline/article/view/163
Description
Summary:Phenol treatment was applied for isolation of rare Actinomycetes using 25 soil samples collected from Pulau Seribu, Tanjung Redep, Manokwari, and Halmahera. The samples were air-dried and suspended in 1.5 % (w/v) phenol solution at 30 oC for 30 minutes, and subsequently cultured on plates of humic acid-vitamin agar (HVA) medium supplemented with cycloheximide (50 μg/mL) and nystatin (50 μg/mL). A total of 61 isolates were obtained and the most dominant isolates were not Streptomyces (only 24.6%), whereas other genera such as Micromonospora, Actinomadura, Microbispora and Polymorphospora were isolated with ratios of 49.2%, 13.1%, 9.8%, and 3.3%, respectively.
ISSN:1978-3477
2087-8575

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