Automatic Quantification of Immunohistochemically Stained Cell Nuclei Based on Standard Reference Cells
A fully automatic method for quantification of images of immunohistochemically stained cell nuclei by computing area proportions, is presented. Agarose embedded cultured fibroblasts were fixed, paraffin embedded and sectioned at 4 µm. They were then stained together with 4 µm sections of the test sp...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Hindawi Limited
1998-01-01
|
Series: | Analytical Cellular Pathology |
Online Access: | http://dx.doi.org/10.1155/1998/195432 |
Summary: | A fully automatic method for quantification of images of immunohistochemically stained cell nuclei by computing area proportions, is presented. Agarose embedded cultured fibroblasts were fixed, paraffin embedded and sectioned at 4 µm. They were then stained together with 4 µm sections of the test specimen obtained from bladder cancer material. |
---|---|
ISSN: | 0921-8912 1878-3651 |