Effect of Mesenchymal Stem Cells treated with 17β-estradiol on the Pattern of Intrinsic Immunity Responses in Collagen-Induced Rheumatoid Arthritis in Wistar Rats

• Main Authors: M Jahan Tigh, SM Abtahi Froshani, N Afzal Ahangaran
• Format: Article
• Language: fas
• Published: Yasuj University Of Medical Sciences 2018-04-01
• Series: Armaghane Danesh Bimonthly Journal
• Subjects: 17 beta-estradiol; mesenchymal stem cells; rheumatoid arthritis; inherent immunity
• Online Access: http://armaghanj.yums.ac.ir/article-1-1926-en.html

Background and Aim: So far, no information has been found on the role of estradiol in altering the immunosuppression of immune system in the in vitro model. The aim of the present study was to evaluate the effects of treatment with 17-beta estradiol MSCs in the regulation of intrinsic immune responses in rheumatoid arthritis (RA) animal models.   Methods: In this experimental study, mesenchymal stem cells were admixed with 17 β-estradiol (E2 100 μM) for 24 hours, and rheumatoid arthritis was induced by collagen and complete perfusion adjuvant in Wistar rats. One week After immunization, rats in untreated groups treated with MSCs without treatment (intraperitoneal injection of two million cells), treated with MSCs treated with E2, changing the diameter of the wrists and the foot of each rat until day 33 After induction, the disease was recorded every 5 days. Data were analyzed by one-way ANOVA and one-way ANOVA. Which were analyzed.   Results: The amount of edema and swelling of the palms of the hands and feet on the last day was positive in the control group (5.9 ± 0.6 mm) more than the treatment groups. Inflation rate in the treated group with E2 treated MSCs (1.69 ± 0.45 mm) was significantly lower than that treated with MSCs without treatment (2.96 ± 0.3 mm). Also, the respiratory burst ability (1. 57 ± 0.11), the amount of phagocytosis (0.11 ± 0.90) and the nitric oxide production (219.66 ± 8.18 μmol / L) in the intrinsic immune cells of the spleen in The positive control group was more than the control group. The rate of respiratory burst, phagocytosis and nitric oxide production in treated MSCs were (0.79 ± 0.99, 0.61 ± 0.08, 155.9 ± 13.29 μM), respectively, and in The group receiving untreated MSCs was 0.99 ± 0.08, 0.88 ± 0.07 and 18.79 ± 12.18 μM respectively.   Conclusion: Treatment of MSCs with 17 beta-estradiol increases regulatory function and inhibitory effect on inflammatory mediators of spleen inherent immune cells in the RA model compared to non-treated mesenchymal stem cells.