Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay

Wild animals, such as rodents seem to be competent reservoir of bacteria-borne zoonotic diseases which disseminate in human. We investigated the presence of E. coli, Shiga toxin-producing E. coli (STEC), and Salmonella in the feces of six category wild rodent species (Apodemus agrarius, A. peninsula...

Full description

Bibliographic Details
Main Authors: Md Mafizur Rahman, Sang Jin Lim, Wook Han Kim, Jae Youl Cho, Yung Chul Park
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:Data in Brief
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2352340920313214
id doaj-ae2ca3e7d0874036958a27d7d1126348
record_format Article
spelling doaj-ae2ca3e7d0874036958a27d7d11263482020-12-21T04:44:23ZengElsevierData in Brief2352-34092020-12-0133106439Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assayMd Mafizur Rahman0Sang Jin Lim1Wook Han Kim2Jae Youl Cho3Yung Chul Park4Division of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea; Faculty of Biological Scienec, Dept. of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, BangladeshDivision of Forest Science, Kangwon National University, Chuncheon 24341, Republic of KoreaNational Institute of Crop Science, Rural Development Administration, Jeonju, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Chunchun-Dong 300, Suwon 440-746, Republic of Korea; Co-corresponding author: Jae Youl ChoDivision of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea; Corresponding author: Yung Chul ParkWild animals, such as rodents seem to be competent reservoir of bacteria-borne zoonotic diseases which disseminate in human. We investigated the presence of E. coli, Shiga toxin-producing E. coli (STEC), and Salmonella in the feces of six category wild rodent species (Apodemus agrarius, A. peninsulae, A. sylvaticus, Micromys minutus, Myodes regulus, and R. norvegicus) captured from different agricultural regions in South Korea. Among them, A. agrarius, which account for 65% of total (N = 52) individuals, are most widely distributed and abundant in various agroecosystems in South Korea. The bacterial identification was performed by cultural and molecular methods. In cultural method, the fecal cultures from 26 individuals formed colonies on E. coli-selective EMB agar media. Of them, the fecal cultures from 18 individuals also produced colonies on the Shiga toxin-producing E. coli-selective CT-SMAC agar media as well as the EMB agar media. In molecular method, polymerase chain reaction (PCR) was carried out to detect two virulence genes (stx1 and stx2) of isolated E. coli. The amplified dataset of stx1 and stx2 genes of E. coli were sequenced. In this manuscript, E. coli and STEC were detected but there were no Salmonella species. The wild rodents’ data would provide important information on reservoirs of those pathogenic bacteria.http://www.sciencedirect.com/science/article/pii/S2352340920313214WildlifeApodemus agrariusE. coliShiga toxin-producing E. coliVirulence genes
collection DOAJ
language English
format Article
sources DOAJ
author Md Mafizur Rahman
Sang Jin Lim
Wook Han Kim
Jae Youl Cho
Yung Chul Park
spellingShingle Md Mafizur Rahman
Sang Jin Lim
Wook Han Kim
Jae Youl Cho
Yung Chul Park
Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay
Data in Brief
Wildlife
Apodemus agrarius
E. coli
Shiga toxin-producing E. coli
Virulence genes
author_facet Md Mafizur Rahman
Sang Jin Lim
Wook Han Kim
Jae Youl Cho
Yung Chul Park
author_sort Md Mafizur Rahman
title Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay
title_short Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay
title_full Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay
title_fullStr Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay
title_full_unstemmed Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay
title_sort prevalence data of diarrheagenic e. coli in the fecal pellets of wild rodents using culture methods and pcr assay
publisher Elsevier
series Data in Brief
issn 2352-3409
publishDate 2020-12-01
description Wild animals, such as rodents seem to be competent reservoir of bacteria-borne zoonotic diseases which disseminate in human. We investigated the presence of E. coli, Shiga toxin-producing E. coli (STEC), and Salmonella in the feces of six category wild rodent species (Apodemus agrarius, A. peninsulae, A. sylvaticus, Micromys minutus, Myodes regulus, and R. norvegicus) captured from different agricultural regions in South Korea. Among them, A. agrarius, which account for 65% of total (N = 52) individuals, are most widely distributed and abundant in various agroecosystems in South Korea. The bacterial identification was performed by cultural and molecular methods. In cultural method, the fecal cultures from 26 individuals formed colonies on E. coli-selective EMB agar media. Of them, the fecal cultures from 18 individuals also produced colonies on the Shiga toxin-producing E. coli-selective CT-SMAC agar media as well as the EMB agar media. In molecular method, polymerase chain reaction (PCR) was carried out to detect two virulence genes (stx1 and stx2) of isolated E. coli. The amplified dataset of stx1 and stx2 genes of E. coli were sequenced. In this manuscript, E. coli and STEC were detected but there were no Salmonella species. The wild rodents’ data would provide important information on reservoirs of those pathogenic bacteria.
topic Wildlife
Apodemus agrarius
E. coli
Shiga toxin-producing E. coli
Virulence genes
url http://www.sciencedirect.com/science/article/pii/S2352340920313214
work_keys_str_mv AT mdmafizurrahman prevalencedataofdiarrheagenicecoliinthefecalpelletsofwildrodentsusingculturemethodsandpcrassay
AT sangjinlim prevalencedataofdiarrheagenicecoliinthefecalpelletsofwildrodentsusingculturemethodsandpcrassay
AT wookhankim prevalencedataofdiarrheagenicecoliinthefecalpelletsofwildrodentsusingculturemethodsandpcrassay
AT jaeyoulcho prevalencedataofdiarrheagenicecoliinthefecalpelletsofwildrodentsusingculturemethodsandpcrassay
AT yungchulpark prevalencedataofdiarrheagenicecoliinthefecalpelletsofwildrodentsusingculturemethodsandpcrassay
_version_ 1724375819994791936