Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay
Wild animals, such as rodents seem to be competent reservoir of bacteria-borne zoonotic diseases which disseminate in human. We investigated the presence of E. coli, Shiga toxin-producing E. coli (STEC), and Salmonella in the feces of six category wild rodent species (Apodemus agrarius, A. peninsula...
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doaj-ae2ca3e7d0874036958a27d7d11263482020-12-21T04:44:23ZengElsevierData in Brief2352-34092020-12-0133106439Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assayMd Mafizur Rahman0Sang Jin Lim1Wook Han Kim2Jae Youl Cho3Yung Chul Park4Division of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea; Faculty of Biological Scienec, Dept. of Biotechnology and Genetic Engineering, Islamic University, Kushtia 7003, BangladeshDivision of Forest Science, Kangwon National University, Chuncheon 24341, Republic of KoreaNational Institute of Crop Science, Rural Development Administration, Jeonju, Republic of KoreaDepartment of Genetic Engineering, Sungkyunkwan University, Chunchun-Dong 300, Suwon 440-746, Republic of Korea; Co-corresponding author: Jae Youl ChoDivision of Forest Science, Kangwon National University, Chuncheon 24341, Republic of Korea; Corresponding author: Yung Chul ParkWild animals, such as rodents seem to be competent reservoir of bacteria-borne zoonotic diseases which disseminate in human. We investigated the presence of E. coli, Shiga toxin-producing E. coli (STEC), and Salmonella in the feces of six category wild rodent species (Apodemus agrarius, A. peninsulae, A. sylvaticus, Micromys minutus, Myodes regulus, and R. norvegicus) captured from different agricultural regions in South Korea. Among them, A. agrarius, which account for 65% of total (N = 52) individuals, are most widely distributed and abundant in various agroecosystems in South Korea. The bacterial identification was performed by cultural and molecular methods. In cultural method, the fecal cultures from 26 individuals formed colonies on E. coli-selective EMB agar media. Of them, the fecal cultures from 18 individuals also produced colonies on the Shiga toxin-producing E. coli-selective CT-SMAC agar media as well as the EMB agar media. In molecular method, polymerase chain reaction (PCR) was carried out to detect two virulence genes (stx1 and stx2) of isolated E. coli. The amplified dataset of stx1 and stx2 genes of E. coli were sequenced. In this manuscript, E. coli and STEC were detected but there were no Salmonella species. The wild rodents’ data would provide important information on reservoirs of those pathogenic bacteria.http://www.sciencedirect.com/science/article/pii/S2352340920313214WildlifeApodemus agrariusE. coliShiga toxin-producing E. coliVirulence genes |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Md Mafizur Rahman Sang Jin Lim Wook Han Kim Jae Youl Cho Yung Chul Park |
spellingShingle |
Md Mafizur Rahman Sang Jin Lim Wook Han Kim Jae Youl Cho Yung Chul Park Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay Data in Brief Wildlife Apodemus agrarius E. coli Shiga toxin-producing E. coli Virulence genes |
author_facet |
Md Mafizur Rahman Sang Jin Lim Wook Han Kim Jae Youl Cho Yung Chul Park |
author_sort |
Md Mafizur Rahman |
title |
Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay |
title_short |
Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay |
title_full |
Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay |
title_fullStr |
Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay |
title_full_unstemmed |
Prevalence data of diarrheagenic E. coli in the fecal pellets of wild rodents using culture methods and PCR assay |
title_sort |
prevalence data of diarrheagenic e. coli in the fecal pellets of wild rodents using culture methods and pcr assay |
publisher |
Elsevier |
series |
Data in Brief |
issn |
2352-3409 |
publishDate |
2020-12-01 |
description |
Wild animals, such as rodents seem to be competent reservoir of bacteria-borne zoonotic diseases which disseminate in human. We investigated the presence of E. coli, Shiga toxin-producing E. coli (STEC), and Salmonella in the feces of six category wild rodent species (Apodemus agrarius, A. peninsulae, A. sylvaticus, Micromys minutus, Myodes regulus, and R. norvegicus) captured from different agricultural regions in South Korea. Among them, A. agrarius, which account for 65% of total (N = 52) individuals, are most widely distributed and abundant in various agroecosystems in South Korea. The bacterial identification was performed by cultural and molecular methods. In cultural method, the fecal cultures from 26 individuals formed colonies on E. coli-selective EMB agar media. Of them, the fecal cultures from 18 individuals also produced colonies on the Shiga toxin-producing E. coli-selective CT-SMAC agar media as well as the EMB agar media. In molecular method, polymerase chain reaction (PCR) was carried out to detect two virulence genes (stx1 and stx2) of isolated E. coli. The amplified dataset of stx1 and stx2 genes of E. coli were sequenced. In this manuscript, E. coli and STEC were detected but there were no Salmonella species. The wild rodents’ data would provide important information on reservoirs of those pathogenic bacteria. |
topic |
Wildlife Apodemus agrarius E. coli Shiga toxin-producing E. coli Virulence genes |
url |
http://www.sciencedirect.com/science/article/pii/S2352340920313214 |
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