Characterization of the Clinical Significance of PD-1/PD-Ls Expression and Methylation in Patients With Low-Grade Glioma

Background: Immune checkpoints play crucial roles in the immune escape of cancer cells. However, the exact prognostic values of expression and methylation of programmed-death 1 (PD-1), programmed-death-ligand 1 (PD-L1) and PD-L2 in low-grade glioma (LGG) have not been well-defined yet. Methods: A to...

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Main Authors: Jie Mei, Yun Cai, Rui Xu, Xuejing Yang, Weijian Zhou, Huiyu Wang, Chaoying Liu
Format: Article
Language:English
Published: SAGE Publishing 2021-05-01
Series:Technology in Cancer Research & Treatment
Online Access:https://doi.org/10.1177/15330338211011970
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spelling doaj-ae1533bd606a4fd3bd94c5c0127785c02021-05-10T03:33:32ZengSAGE PublishingTechnology in Cancer Research & Treatment1533-03382021-05-012010.1177/15330338211011970Characterization of the Clinical Significance of PD-1/PD-Ls Expression and Methylation in Patients With Low-Grade GliomaJie Mei0Yun Cai1Rui Xu2Xuejing Yang3Weijian Zhou4Huiyu Wang5Chaoying Liu6 Department of Oncology, , Wuxi, China Department of Bioinformatics, , Nanjing, China School of Basic Medical Sciences, , Nanjing, China Department of Oncology, , Wuxi, China Department of Oncology, , Wuxi, China Department of Oncology, , Wuxi, China Department of Oncology, , Wuxi, ChinaBackground: Immune checkpoints play crucial roles in the immune escape of cancer cells. However, the exact prognostic values of expression and methylation of programmed-death 1 (PD-1), programmed-death-ligand 1 (PD-L1) and PD-L2 in low-grade glioma (LGG) have not been well-defined yet. Methods: A total 514 LGG samples from the Cancer Genome Atlas (TCGA) dataset containing gene expression, DNA methylation, and survival data were enrolled in our study. Besides, a total of 137 primary LGG samples from the Chinese Glioma Genome Atlas (CGGA) database were also extracted for the survival analysis of the prognostic values of PD-1/PD-Ls expression. Results: PD-1/PD-Ls had distinct co-expression patterns in LGG tissues. The expression and methylation level of PD-1/PD-Ls seemed to be various in different LGG subtypes. Besides, overexpression and hypo-methylation of PD-1/PD-Ls were associated with worse prognosis. In addition, PD-1/PD-Ls expression was positively associated with TIICs infiltration, while their methylation was negatively associated with TIICs infiltration. Moreover, PD-1/PD-Ls and their positively correlated gene mainly participated in immune response related biological processes. Conclusion: To conclude, overexpression and hypo-methylation of PD-1/PD-Ls predicted unfavorable prognosis in LGG patients, suggesting those patients may benefit from PD1/PD-Ls checkpoint inhibitors treatment.https://doi.org/10.1177/15330338211011970
collection DOAJ
language English
format Article
sources DOAJ
author Jie Mei
Yun Cai
Rui Xu
Xuejing Yang
Weijian Zhou
Huiyu Wang
Chaoying Liu
spellingShingle Jie Mei
Yun Cai
Rui Xu
Xuejing Yang
Weijian Zhou
Huiyu Wang
Chaoying Liu
Characterization of the Clinical Significance of PD-1/PD-Ls Expression and Methylation in Patients With Low-Grade Glioma
Technology in Cancer Research & Treatment
author_facet Jie Mei
Yun Cai
Rui Xu
Xuejing Yang
Weijian Zhou
Huiyu Wang
Chaoying Liu
author_sort Jie Mei
title Characterization of the Clinical Significance of PD-1/PD-Ls Expression and Methylation in Patients With Low-Grade Glioma
title_short Characterization of the Clinical Significance of PD-1/PD-Ls Expression and Methylation in Patients With Low-Grade Glioma
title_full Characterization of the Clinical Significance of PD-1/PD-Ls Expression and Methylation in Patients With Low-Grade Glioma
title_fullStr Characterization of the Clinical Significance of PD-1/PD-Ls Expression and Methylation in Patients With Low-Grade Glioma
title_full_unstemmed Characterization of the Clinical Significance of PD-1/PD-Ls Expression and Methylation in Patients With Low-Grade Glioma
title_sort characterization of the clinical significance of pd-1/pd-ls expression and methylation in patients with low-grade glioma
publisher SAGE Publishing
series Technology in Cancer Research & Treatment
issn 1533-0338
publishDate 2021-05-01
description Background: Immune checkpoints play crucial roles in the immune escape of cancer cells. However, the exact prognostic values of expression and methylation of programmed-death 1 (PD-1), programmed-death-ligand 1 (PD-L1) and PD-L2 in low-grade glioma (LGG) have not been well-defined yet. Methods: A total 514 LGG samples from the Cancer Genome Atlas (TCGA) dataset containing gene expression, DNA methylation, and survival data were enrolled in our study. Besides, a total of 137 primary LGG samples from the Chinese Glioma Genome Atlas (CGGA) database were also extracted for the survival analysis of the prognostic values of PD-1/PD-Ls expression. Results: PD-1/PD-Ls had distinct co-expression patterns in LGG tissues. The expression and methylation level of PD-1/PD-Ls seemed to be various in different LGG subtypes. Besides, overexpression and hypo-methylation of PD-1/PD-Ls were associated with worse prognosis. In addition, PD-1/PD-Ls expression was positively associated with TIICs infiltration, while their methylation was negatively associated with TIICs infiltration. Moreover, PD-1/PD-Ls and their positively correlated gene mainly participated in immune response related biological processes. Conclusion: To conclude, overexpression and hypo-methylation of PD-1/PD-Ls predicted unfavorable prognosis in LGG patients, suggesting those patients may benefit from PD1/PD-Ls checkpoint inhibitors treatment.
url https://doi.org/10.1177/15330338211011970
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