Proteomic insights into non-small cell lung cancer: New ideas for cancer diagnosis and therapy from a functional viewpoint

• Main Authors: Johannes Linxweiler, Laxmikanth Kollipara, René P. Zahedi, Pavel Lampel, Richard Zimmermann, Markus Greiner
• Format: Article
• Language: English
• Published: Elsevier 2014-09-01
• Series: EuPA Open Proteomics
• Subjects: Non-small cell lung cancer (NSCLC); Sec62; 2D-DIGE; Vimentin; Plastin 3; Epithelial-mesenchymal transition (EMT)
• Online Access: http://www.sciencedirect.com/science/article/pii/S2212968514000373
• ISSN: 2212-9685

We recently characterized SEC62 as an oncogene in non-small-cell lung cancer (NSCLC). Here we aimed to gain further insight into the molecular mechanisms of the cancer-related functions of this oncogene. We performed 2D-DIGE proteome analysis of tumor material from patients with NSCLC and of HEK293 cells stably overexpressing plasmid-encoded SEC62, combined with investigation of the Sec62 interactome. Furthermore, we analyzed the proteomic effects of siRNA-mediated depletion of the Sec62-interacting protein Sec63. We identified a comprehensive list of differentially regulated proteins, providing new insights into the molecular mechanisms of the cancer-related functions of Sec62 in cell migration, drug resistance, and Ca2+-homeostasis.