The relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different diets

<p>Archaeol is a cell membrane lipid of methanogenic archaea excreted in feces and is therefore a potential biomarker for individual methane emission (MEM). The aims of this study were to examine the potential of the fecal archaeol concentration (fArch) to be a proxy for MEM prediction in cows...

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Main Authors: L.-M. Sandberg, G. Thaller, S. Görs, B. Kuhla, C. C. Metges, N. Krattenmacher
Format: Article
Language:English
Published: Copernicus Publications 2020-07-01
Series:Archives Animal Breeding
Online Access:https://aab.copernicus.org/articles/63/211/2020/aab-63-211-2020.pdf
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spelling doaj-adf3ae1589d846a2bb2e8bd3ef0462202020-11-25T03:00:30ZengCopernicus PublicationsArchives Animal Breeding0003-94382363-98222020-07-016321121810.5194/aab-63-211-2020The relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different dietsL.-M. Sandberg0G. Thaller1S. Görs2B. Kuhla3C. C. Metges4C. C. Metges5N. Krattenmacher6Institute of Animal Breeding and Husbandry, Kiel University, Kiel, 24098, GermanyInstitute of Animal Breeding and Husbandry, Kiel University, Kiel, 24098, GermanyInstitute of Nutritional Physiology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, 18196, GermanyInstitute of Nutritional Physiology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, 18196, GermanyInstitute of Nutritional Physiology, Leibniz Institute for Farm Animal Biology (FBN), Dummerstorf, 18196, GermanyChair of Nutritional Physiology and Animal Nutrition, Faculty of Agricultural and Environmental Sciences, University of Rostock, Rostock, 18059, GermanyInstitute of Animal Breeding and Husbandry, Kiel University, Kiel, 24098, Germany<p>Archaeol is a cell membrane lipid of methanogenic archaea excreted in feces and is therefore a potential biomarker for individual methane emission (MEM). The aims of this study were to examine the potential of the fecal archaeol concentration (fArch) to be a proxy for MEM prediction in cows fed different diets and determine if the time of fecal collection affected the archaeol concentration. Thus, we investigated (i) the variation of the fArch concentration in spot samples of feces taken thrice within 8&thinsp;h during respiration chamber measurements and (ii) the effect of two diets differing in nutrient composition and net energy content on the relationship between fArch and MEM in lactating cows. Two consecutive respiration trials with four primiparous and six multiparous lactating Holstein cows were performed. In the first trial (T1) at <span class="inline-formula">100±3</span>&thinsp;d in milk (IM), a diet moderate in starch and fat content was fed for ad libitum intake, whereas in the second trial (T2) at <span class="inline-formula">135±3</span>&thinsp;d IM, cows received a diet lower in starch and fat. Individual MEM (g&thinsp;d<span class="inline-formula"><sup>−1</sup></span>) was measured for 24&thinsp;h. Fecal samples were taken at 06:30, 10:00, and 14:30&thinsp;LT and analyzed for fArch using Soxhlet lipid extraction and GC–MS. Cows produced less methane (364&thinsp;g&thinsp;<span class="inline-formula">CH<sub>4</sub></span>&thinsp;d<span class="inline-formula"><sup>−1</sup></span>) during T1 and had significantly lower fArch concentrations (37.1&thinsp;<span class="inline-formula">µ</span>g&thinsp;g<span class="inline-formula"><sup>−1</sup></span> dry matter; DM) compared to T2 (392&thinsp;g&thinsp;<span class="inline-formula">CH<sub>4</sub></span>&thinsp;d<span class="inline-formula"><sup>−1</sup></span> and 47.6&thinsp;<span class="inline-formula">µ</span>g&thinsp;g<span class="inline-formula"><sup>−1</sup></span> DM). A significant positive relationship between fArch (<span class="inline-formula">µ</span>g&thinsp;g<span class="inline-formula"><sup>−1</sup></span> fecal DM) and MEM, expressed on a dry matter intake (DMI) basis (g&thinsp;kg<span class="inline-formula"><sup>−1</sup></span> DMI), was found (<span class="inline-formula"><i>R</i><sup>2</sup>=0.53</span>, <span class="inline-formula"><i>n</i>=20</span>). Among samples collected over the day, those collected at 10:00&thinsp;LT provided the best coefficient of determination for MEM (<span class="inline-formula"><i>R</i><sup>2</sup>=0.23</span>). In conclusion, fArch offers some potential in serving as a proxy for innovative breeding schemes to lower enteric methane when fecal samples are taken at a certain time of the day, but more data on the sources of variation of the MEM&thinsp;<span class="inline-formula">:</span>&thinsp;fArch ratios are required.</p>https://aab.copernicus.org/articles/63/211/2020/aab-63-211-2020.pdf
collection DOAJ
language English
format Article
sources DOAJ
author L.-M. Sandberg
G. Thaller
S. Görs
B. Kuhla
C. C. Metges
C. C. Metges
N. Krattenmacher
spellingShingle L.-M. Sandberg
G. Thaller
S. Görs
B. Kuhla
C. C. Metges
C. C. Metges
N. Krattenmacher
The relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different diets
Archives Animal Breeding
author_facet L.-M. Sandberg
G. Thaller
S. Görs
B. Kuhla
C. C. Metges
C. C. Metges
N. Krattenmacher
author_sort L.-M. Sandberg
title The relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different diets
title_short The relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different diets
title_full The relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different diets
title_fullStr The relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different diets
title_full_unstemmed The relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different diets
title_sort relationship between methane emission and daytime-dependent fecal archaeol concentration in lactating dairy cows fed two different diets
publisher Copernicus Publications
series Archives Animal Breeding
issn 0003-9438
2363-9822
publishDate 2020-07-01
description <p>Archaeol is a cell membrane lipid of methanogenic archaea excreted in feces and is therefore a potential biomarker for individual methane emission (MEM). The aims of this study were to examine the potential of the fecal archaeol concentration (fArch) to be a proxy for MEM prediction in cows fed different diets and determine if the time of fecal collection affected the archaeol concentration. Thus, we investigated (i) the variation of the fArch concentration in spot samples of feces taken thrice within 8&thinsp;h during respiration chamber measurements and (ii) the effect of two diets differing in nutrient composition and net energy content on the relationship between fArch and MEM in lactating cows. Two consecutive respiration trials with four primiparous and six multiparous lactating Holstein cows were performed. In the first trial (T1) at <span class="inline-formula">100±3</span>&thinsp;d in milk (IM), a diet moderate in starch and fat content was fed for ad libitum intake, whereas in the second trial (T2) at <span class="inline-formula">135±3</span>&thinsp;d IM, cows received a diet lower in starch and fat. Individual MEM (g&thinsp;d<span class="inline-formula"><sup>−1</sup></span>) was measured for 24&thinsp;h. Fecal samples were taken at 06:30, 10:00, and 14:30&thinsp;LT and analyzed for fArch using Soxhlet lipid extraction and GC–MS. Cows produced less methane (364&thinsp;g&thinsp;<span class="inline-formula">CH<sub>4</sub></span>&thinsp;d<span class="inline-formula"><sup>−1</sup></span>) during T1 and had significantly lower fArch concentrations (37.1&thinsp;<span class="inline-formula">µ</span>g&thinsp;g<span class="inline-formula"><sup>−1</sup></span> dry matter; DM) compared to T2 (392&thinsp;g&thinsp;<span class="inline-formula">CH<sub>4</sub></span>&thinsp;d<span class="inline-formula"><sup>−1</sup></span> and 47.6&thinsp;<span class="inline-formula">µ</span>g&thinsp;g<span class="inline-formula"><sup>−1</sup></span> DM). A significant positive relationship between fArch (<span class="inline-formula">µ</span>g&thinsp;g<span class="inline-formula"><sup>−1</sup></span> fecal DM) and MEM, expressed on a dry matter intake (DMI) basis (g&thinsp;kg<span class="inline-formula"><sup>−1</sup></span> DMI), was found (<span class="inline-formula"><i>R</i><sup>2</sup>=0.53</span>, <span class="inline-formula"><i>n</i>=20</span>). Among samples collected over the day, those collected at 10:00&thinsp;LT provided the best coefficient of determination for MEM (<span class="inline-formula"><i>R</i><sup>2</sup>=0.23</span>). In conclusion, fArch offers some potential in serving as a proxy for innovative breeding schemes to lower enteric methane when fecal samples are taken at a certain time of the day, but more data on the sources of variation of the MEM&thinsp;<span class="inline-formula">:</span>&thinsp;fArch ratios are required.</p>
url https://aab.copernicus.org/articles/63/211/2020/aab-63-211-2020.pdf
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